Citrus genus is the most important fruit tree crop in the world and lemon is the third most important Citrus species. The evaluation of the potential inhibitory activity on α-amylase and pancreatic lipase by Citrus lemon fruits of Algerian origin aiming for new applications of the fruits in nutrition and health was carried out. Lemon fruit EtOAc (ethyl acetate) extract showed higher contents of phytochemicals such as total phenolics and flavonoids. Furthermore, lemon fruit showed higher potential for inhibitory effects on α-amylase and pancreatic lipase. It could be concluded that lemon fruits are of great value for nutrition and treatment of diet-related diseases such as obesity and diabetes.