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Magnetic nanoparticles with green cover sodium alginate and Ag, MnCoFe2O4@Sodium alginate/Ag (MCF@S-ALG/Ag) MNPs, were prepared by a simple and clean method from Sargassum Vulgare brown algae. The structure of these nanoparticles was characterized by the Fourier transform infrared (FT-IR), X-ray powder diffraction (XRD), field emission-scanning electron microscope (FE-SEM), energy-dispersive X-ray spectroscopy (EDX), thermogravimetric analysis (TGA), and vibrating sample magnetometer (VSM). Furthermore, the antibacterial activity of MCF@S-ALG/Ag MNPs was tested for two bacterial of gram-negative (Escherichia coli (E. coli)) bacteria and gram-positive (Staphylococcus aureus (S. aureus)) bacteria. The MCF@S-ALG/Ag MNPs showed the inhibition zone 16.32 mm for S. aureus and 12.84 mm for E. coli bacteria. The minimal inhibitory concentration (MIC) and the minimal bactericidal concentration (MBC) of MCF@S-ALG/Ag MNPs for S. aureus bacteria were found 20 µg/mL and 40µg/mL, respectively.

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