No abstract

Thermophilic bacteria are a source of bioactive compounds that have many benefits for human life. One of them is as a source of antimicrobials. This research aimed to identify and characterize the promising thermophilic bacterial isolates by analyzing bioactive compounds and their potential as antimicrobial agents. Thermophilic bacteria with the code LBKURCC were taken from the collection of the Biochemistry Laboratory of the University of Riau. Forty-four purified strains of thermophilic bacteria were tested for antimicrobial ability. These thermophilic bacteria were taken from hot springs located in the Sumatra provinces of West Sumatra and Riau. Strain LBKURCC218 isolated from Rimbo Panti hot springs in West Sumatra was chosen to further investigate antimicrobials production. Isolates of hot spring bacteria that produced the highest antimicrobial were identified by comparing the similarity of the 16S rRNA gene sequences. BLAST result and phylogenetic tree showed that the selected thermophilic bacterial strain was similar to Bacillus paramicoides with the similarity index of 99.93%. Analysis of bioactive compounds of the ethyl acetate extract of liquid cultures of B. paramycoides LBKURCC218 showed the best producer of antimicrobial compounds compared to other isolates. The most identified compounds from the ethyl acetate extract were Dodecanoic acid, representing 23.62% of the total compounds, followed by 11-Dodecanoic acid at 17.84%. Ethyl acetate extract of B. paramycoides LBKURCC218 has a high inhibition zone against Escherichia coli, Staphylococcus aureus, and Candida albicans.

Recurrent miscarriage can be defined as two or more consequence unsuccessful clinical pregnancies and confirmed by ultrasonography or histopathological examination and consider the most common clinical complications reproduction, and it affects (2%-5%) of reproduction couple. The present study included 140 women who were already diagnosed with recurrent miscarriage. Following complete clinical workup, including history, examination and investigations, enrolled women were classified into two main subgroups, explained group (n = 85) and unexplained group (n = 55). In this study, the result showed no significant difference found between the two subgroups explained and unexplained regarding age and the body mass index. In the obstetric history characteristics of women enrolled in the current study, there is no significant difference between the explained and unexplained group according to gravity, parity, and the number of abortions. Additionally, no significant difference according to gestational age and types of abortion but found significant in the type of complete abortion between the explained and unexplained group. Results showed that recurrent miscarriage rates increased in women with positive anti-phospholipids, in addition, a significant difference in the type of complete abortion between the explained and unexplained group of recurrent miscarriage women.