Salinity stress is a barrier to crop production, quality yield, and sustainable agriculture. The current study investigated the plant growth promotion, biochemical and molecular characterization of bacterial strain Enterobacter cloacae PM23 under salinity stress (i.e., 0, 300, 600, and 900 mM). E. cloacae PM23 showed tolerance of up to 3 M NaCl when subjected to salinity stress. Antibiotic-resistant Iturin C (ItuC) and bio-surfactant-producing genes (sfp and srfAA) were amplified in E. cloacae PM23, indicating its multi-stress resistance potential under biotic and abiotic stresses. Moreover, the upregulation of stress-related genes (APX and SOD) helped to mitigate salinity stress and improved plant growth. Inoculation of E. cloacae PM23 enhanced plant growth, biomass, and photosynthetic pigments under salinity stress. Bacterial strain E. cloacae PM23 showed distinctive salinity tolerance and plant growth-promoting traits such as indole-3-acetic acid (IAA), siderophore, ACC deaminase, and exopolysaccharides production under salinity stress. To alleviate salinity stress, E. cloacae PM23 inoculation enhanced radical scavenging capacity, relative water content, soluble sugars, proteins, total phenolic, and flavonoid content in maize compared to uninoculated (control) plants. Moreover, elevated levels of antioxidant enzymes and osmoprotectants (Free amino acids, glycine betaine, and proline) were noticed in E. cloacae PM23 inoculated plants compared to control plants. The inoculation of E. cloacae PM23 significantly reduced oxidative stress markers under salinity stress. These findings suggest that multi-stress tolerant E. cloacae PM23 could enhance plant growth by mitigating salt stress and provide a baseline and ecofriendly approach to address salinity stress for sustainable agriculture.

No abstract

Protein phosphatase 2Cs (PP2Cs) belong to the largest protein phosphatase family in plants. Some members have been described as being negative modulators of plant growth and development, as well as responses to hormones and environmental stimuli. However, little is known about the members of PP2C clade D, which may be involved in the regulation of signaling pathways, especially in response to saline and alkali stresses. Here, we identified 13 PP2C orthologs from the wild soybean (Glycine soja) genome. We examined the sequence characteristics, chromosome locations and duplications, gene structures, and promoter cis-elements of the PP2C clade D genes in Arabidopsis and wild soybean. Our results showed that GsPP2C clade D (GsAPD) genes exhibit more gene duplications than AtPP2C clade D genes. Plant hormone and abiotic stress-responsive elements were identified in the promoter regions of most PP2C genes. Moreover, we investigated their expression patterns in roots, stems, and leaves. Quantitative real-time PCR analyses revealed that the expression levels of representative GsPP2C and AtPP2C clade D genes were significantly influenced by alkali and salt stresses, suggesting that these genes might be associated with or directly involved in the relevant stress signaling pathways. Our results established a foundation for further functional characterization of PP2C clade D genes in the future.

The aim of this study was to determine the attitude of pharmacists and non-pharmacists in Karachi towards the use of non-prescription medicines. A cross-sectional survey was conducted from August 2012 to October 2012 among pharmacist and non-pharmacists. Data was analyzed and results were compared using Chi-square test with the help of statistical software package (SPSS version 19). Overall prevalence of self-medication was found to be 81.28%. Factors like busy life schedule (27.58%), previous experience of medicine (20.68%) and better drug information (20.86%), contributed differently in promoting self medication to pharmacists, whereas busy life schedule (45%), was one of the major factor of self medication among non pharmacist. The major clinical conditions in which self medication was observed in the pharmacists were flu (36.9%), pain (27.59%), fever (25.86%), diarrhea (24.14%) and headache (22.07%), whereas in the non pharmacists, the main conditions were headache (55.71%), diarrhea (53.33%), fever (35.71%) and flu (35.24%). Self-medication was commonly observed both in pharmacist (62.08%) and non pharmacist (71.43%) in Karachi, but there was a significant increase in non pharmacist ( 2 =4.756, p<0.05). In order to control this prevailing problem, legislation are to be made by the government especially to implement and facilitate the prescription system, conducting awareness programs and restricting drug advertisements for public.

No abstract