Introduction: During COVID-19 pandemic, isolation and quarantine as preventive measures have been implemented. Isolation is often a bad experience for isolated people and it could affect the psychological and mental health. Objectives: To study the impact of isolation on the psychological and mental health of isolated hospitalized suspected and confirmed COVID-19 patients. Material & Methods: A single-center cohort, descriptive survey involving isolated confirmed and suspected COVID-19 patients in Qatif central hospital, Eastern province, Saudi Arabia. The study was conducted between March 22 and April 18, 2020. Information was collected through an online Arabic-language questionnaire. Our sample included both male and female. Anxiety and depression symptoms during hospitalization were evaluated with the Hospital Anxiety and Depression Scale. Results: A total of 52 questionnaires were returned completed. The majority of the respondents were females (65.4%). Most of the participants were less than 60 years of age (90.4%). Three patients were known to have previous mental illness. About two-thirds of the participants were isolated in shared rooms. The duration of isolation was less than 10 days in 72.3% of our sample. Borderline and abnormal scores for depression and anxiety were observed in about 38.4%, and 17.3% of patients, respectively. Three patients had both abnormal depression and anxiety scores. Conclusion: Isolation for contagious infections such as COVID-19 could cause How to cite this paper: Alhajjaj, A