Background: The total number of oocytes, oocyte maturation, and oocyte fertilization all significantly affect how successful in vitro fertilization (IVF) is. Additionally, it has been demonstrated that several chemicals in follicular fluid, including cytokines and growth factors, have a major impact on follicular maturation, ovulation, and follicular atresia. One of these molecules, Pregnancy Associated Plasma Protein-A (PAPP-A), a part of the Insulin like Growth Factor (IGF) pathway, has been demonstrated to be generated by granulosa cells and present in follicular fluid. Objective: The aim of this study is to measure the level of Pregnancy Associated Plasma Protein-A (PAPP-A) in Follicular Fluid (FF) of infertile women undergoing ICSI, and to looking into the connections of PAPP-A with some reproductive hormones level. Patients and methods: The study included infertile women from Al-

Background: Pregnancy Associated Placental Protein-A (PAPP-A) is a zinc-binding metalloproteinase with a key role in the insulin-like growth factor (IGF) pathway. The IGF system plays an essential role in follicular development, including steroid hormone synthesis, growth, recruitment, and apoptosis of follicles of women undergoing intracytoplasmic sperm injection (ICSI). Objective: The objective of this study is to measure Pregnancy Associated Plasma Protein-A (PAPP-A) in serum of infertile women undergoing ICSI, and to investigate the association of PAPP-A with some reproductive hormones level. A total of 45 infertile women. Results:The results of this study was Serum PAPP-A level was not significant with hormones level (FSH, LH PRO and AMH) except E2 hormone was significant. Conclusion: Outcome show that no relation between studied reproductive hormones and serum PAPP-A except E2 levels that are high cause the egg to mature and release from the ovary.