This study is on the multipotential mesenchymal cells which enable us to differentiate chondroblasts in presence of Transforming growth factor (TGFβ) and bone morphogenic factors (BMPs). As use of a transferring system for these growth factors is a notable issue in recent researches, the present study was designed to evaluate the nanosilver transferring system of TGFβ on two sample groups including 42 rats and 4 rabbits as pilot study. The samples were divided in two groups for injection of TGFβ with and without nanosilver. Femoral muscle of rats were injected and then sacrificed after 1, 2 and 4 weeks. This injection was repeated in subperiosteal area of parietal bone of rabbits with decreased dose of nanosilver. The injected areas were examined histopathlogically using H&E and Methylene-blue staining methods. Results revealed chondrogenesis was not detected in all 6 rat groups in all following weeks, but was present in the parietal bone of one rabbit which was injected with TGFβ with nanosilver and sacrificed after 4 weeks. The differences in level of inflammation in and presence of necrosis were significant in TGFβ with nanosilver groups vs TGF ones. Although, addition of nanosilver to TGFβ did not show great cytotoxic responses, it may not have great effects to induction of chondrogenesis in rat femoral muscle. Detection of chondrogenesis in one sample of subperiosteal rabbit parietal bone can be explained by decreased nanosilver dose and difference in anatomic site of injection. So, more investigations need to verify practical use of TGFβ with nanosilver transferring system in repair of chondral defects.