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The laboratory experiment challenges students to determine 6-propyl-2-thiouracil (PTU) based on Prussian blue complex formation. Prussian blue is formed by ferricyanide and Fe­(II) ions which are generated in situ from Fe­(III) ions reduced by PTU. The absorbance of this product was measured at a wavelength of 840 nm, after a reaction time of 30 min. The range of determination was 0.34–3.4 μg mL–1 of PTU per sample. The method was successfully used in an upper-division instrumental analysis laboratory course to determine the concentration of PTU in commercially available pharmaceuticals. Additionally, students in introductory or high school chemistry classes by conducting this experiment will gain a greater understanding of the chemical analysis process, redox reactions, and the Beer–Lambert law.

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