The research included isolation and purification of Trypsin from serum of patient with pancreatic cancer, and estimation its molecular weight by using the two techniques of gel filtration (using sephadex G-50) and electrophoresis (using SDS). Therefore; it was noted that the proteinous peak  isolated from ammonium sulfate solution is saturated (65%) of the serum by using the two techniques gel filtration and electrophoresis had apparent molecular weight (27000) Dalton. The study included (40) patients with pancreas cancer their ages ranged between (30-70) years who attended Al-Jumhuri and Atomic Medicine Hospitals in Mosul; the laboratory examination included estimation levels of: Trypsin, Gamma Glutamyl transferase, Amylase, Paraoxonase  ,Glutathione, Sialic acid and Ceruloplasmin. Results showed that level of Trypsin and Amylase in the serum decreased significantly in  patients with pancreatic cancer in comparison with the control group, but levels of Gamma Glutamyl transferase, paraoxonase, Glutathione, Sialic acid and Ceruloplasmin increased significantly in patients with pancreatic cancer in comparison with the control group.

This study included an estimation for the levels of some biochemical parameters in the serum of those pancreatic cancer patients, by collecting (40) blood samples from patients their ages ranged (30-70) years compared with (55) blood samples of healthy persons as control group. The parameters included Super oxide dismutase, Amylase, Ribonuclease (acidic and alkaline), Arylesterase, Alkaline phosphatease, Carcinoembryonic antigen, carbohydrate antigen 19-9, Total lipid and Bilirubin Results of the Statistical analysis indicated a significant decrease in the activity of Amylase and Super oxide dismutase patients in comparison with the control group. There was a significant increase in the activity of Ribonuclease (acidic and alkaline), Arylesterase, Alkaline phosphatease, Carcinoembryonic antigen, carbohydrate antigen 19-9, Total lipid and Bilirubin in patients in comparison with the control group.