Ganoderma lucidum is a legendary Traditional Chinese Medicine (TCM) over a few thousands of years and one kind of its major active components are Ganoderic acids (GAs). GAs are largely produced in the mushroom primordium and fruiting body but much less in mycelium stage. However, little is known on the underlying regulatory mechanism. As a saprophytic fungus, G. lucidum solely obtains nutrients by wood decaying. Wood in general contains sophisticated chemical components with diverse structural units. To explore a strategy that extensively leads to GAs induction in the submerged liquid fermentation, all chemical components that might be possibly from the wood decaying were tested individually as GAs inducers. It was found that GAs production increased 85.96% by 1.5% microcrystalline cellulose (MCC) and 63.90% by 0.5% D-galactose. The transcription level of a few rate-limiting or chemically diverting enzymes responsible for GAs biosynthesis was greatly induced by MCC and D-galactose. The concentration and time-course titration study indicated that these two chemicals might not be utilized as carbon sources but they played a comprehensive role in the secondary metabolites synthesis. Our data indicated that MCC and D-galactose might be further industrialized for higher GAs production in G. lucidum in submerged fermentation.
Breaking Summer Dormancy of P. ternata 5, 9-trimethyl-12-(1-methylethyl). Our findings show that the summer dormancy of P. ternata which is a naturally evolved trait, can be removed by a single heat responsive gene. The study contributes to generating heat tolerant new Pinellia varieties with enhanced commercially valuable chemicals.
The position of the antheridium on the oogonium of Phytophthora boehmeriae was greatly affected by culture media. Agar medium containing lecithin and basal salts and corn grain agar favored the production of paragynous antheridia, while corn meal agar, lima bean agar, vegetable juice agar, and tomato agar were conducive to formation of amphigynous antheridia. The effect of culture media on antheridial configuration was found to be due to nutrient concentration. A high concentration of nutrients in the media was favored the formation of amphigynous antheridia, and a low concentration of nutrients favored paragynous antheridia.Key words: antheridial configuration, oogonium, nutrients, Phytophthora boehmeriae.
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