Bisphenol A, an endocrine disrupting compounds that affect human homeostasis. Studies on BPA are focusing on the impact of BPA in reproductive function and brain development. However, the effect of BPA on gut especially gastric cells is not well explored. Gut is directly in contact with ingested BPA; therefore, we aimed to determine the effect of BPA exposure on gastric cells proliferation at safe recommended concentration. Human gastric cancer cells (HGC-27) were treated with BPA at different concentration (low: 10-9M, 10-7M; high10-5M, 10-4M) and time point (24 hr, 48 hr, 72 hr). Cell viability assays were determined using MTS assay. Cells were further stained with Alexa Fluor-635 (F-actin) and Fluorescein (Hif-1α) protein for immunocytofluorescence. Data were analysed using ANOVA (p<0.05, n≥3). Cells treated with 10-9M BPA showed significance increase of cell viability after 48 hr (Mean ±SEM; 146%±0.03, p=0.01) and 72 hr (113%±0.03, p=0.00) compared to 24 hr treatment (77%±0.11, p=0.002). Similarly, cell treated with 10-7M BPA showed a significance increase after 48 hr (141%±0.03, p=0.03) and 72 hr (190%±0.03, p=0.02) compared to 24 hr cells treated with 10-7M (88%±0.05, p=0.01) and untreated (100%±0.07). Lower concentration of BPA increases the condensation of F-actin in all HGC-27 cells. Meanwhile, translocation of Hif-1α protein were observed in all BPA-exposed cells. Findings of this study revealed that BPA induced proliferation and condensation of F-actin structure of gastric cancer cells at low concentration.

Extragonadal teratoma of the omentum is a rare clinical entity. We report a case of a large omental teratoma in a postmenopausal woman who presented with 3 days history of right groin pain. She was previously diagnosed with right ovarian teratoma in 2013 but declined surgical intervention. Laparoscopy performed for suspected ovarian cyst torsion discovered a large omental teratoma measuring approximately 11 cm × 9 cm with the concurrent absence of the right fallopian tube and ovary. The mass was removed successfully via laparoscopic approach. The likely mechanism in this case is autoamputation of the adnexae due to chronic ovarian torsion. Establishing correct diagnosis preoperatively is difficult, however an extragonadal teratoma should be one of the differential diagnoses when a round, mobile mass is found in the abdomen. Laparoscopic management is feasible even in the presence of a large extragonadal teratoma.

predict the possibility of advanced gastric cancer patients to receive third-line chemotherapy, based on their baseline characteristics. Methods: We retrospectively collected baseline characteristics and treatment data of 271 patients with unresected advanced gastric cancer who started chemotherapy between January 2006 and June 2017 in Kurashiki Central Hospital. A median age was 68 years, and 190 patients were male. The relationship between patients' characteristics and delivery rate to third-line chemotherapy were investigated. Results: Among the 271 patients, 71 patients (26.2%) received third-line chemotherapy. On univariate analysis, delivery rate to third-line chemotherapy were significantly related to patients' Eastern Cooperative Oncology Group (ECOG) performance status (PS), cancer histology and several laboratory test results at the baseline. Multivariate analysis revealed that PS 0 and serum CRP levels 0.6 mg/dl were independent predictive factors for delivery to third-line chemotherapy; adjusted odds ratio (95% confidence interval) was 4.14 (2.11-8.14) and 2.47 (1 .20-5.11), respectively.

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