Background and Objective: Numerous researches were conducted on the chemical composition of harmala (Péganum hármala), but there are no available comparative studies with a focus on methods and ecology of plants. The chemical composition of the thick carbonic acid extract was studied, based on the ordinary harmala. The objective of the study was to observe the ordinary harmala grown in the central Asia region, which was obtained by the maceration extraction method. Materials and Methods: For this purpose, the chemical composition of the object was determined using a physicochemical method, Gas Chromatography/Mass Spectrometry (GC/MS). This definition is supplemented by a comparative analysis of extracts (alcoholic and carbon dioxide, respectively sample 1 and sample 2) ordinary harmala by their chemical composition. Results: With the above extraction parameter from 2600 g of medicinal plant raw materials, a thick carbon dioxide extract with a total weight of 7.2 g was obtained (samples 1: Alcoholic and sample 2: Carbon dioxide). The yield of the extract is 0.27%. According to the results of carbon dioxide extraction, it was revealed that the most optimal extraction parameter is the extraction parameter of sample 1, due to the high extract yield, 5.1 g (0.19%). Conclusion: Based on the results obtained, prove that carbon dioxide extract is the most optimal for the further development of a medicinal product based on an extract of ordinary harmala with anticholinesterase therapeutic effect.