Development of effective pathogen detection systems is critical for human health. In this study, a micro-gapped interdigitated electrode array-based immunosensor chip is constructed for the detection of a typical pathogen, Escherichia coli (E. coli) O157:H7. The immunosesnor chip was prepared via photolithography and characterized by a digital camera, optical microscope and scanning electron microscope. The E. coli O157:H7 detection mechanism is based on an enzymelinked, sandwiched immunoassay with enzymatic silver deposition. Our results revealed that the proposed immunosensor chip showed selective electrical response towards E. coli O157:H7 over other bacteria including Hemolytic streptococcus, Staphylococcus aureus, Salmonella, Pseudomonas aeruginosa, Chronobacter sakazakii and Bacillus cereus. Our assay exhibited potential practical applications for E. coli O157:H7 determination in water and our methodology might be applied to detect different pathogens by simply selecting specific antibodies. KEYWORDSEscherichia coli O157:H7; micro-gapped interdigitated electrode array; immunoassay; electrochemical sensing Abbreviations AAP ascorbic acid phosphate enzyme ALP alkaline phosphatase APTES 3-aminopropyl triethoxysilane BSA bovine serum albumin EDS energy-dispersive spectroscopy ELISA enzyme-linked immunosorbent assay LSV linear sweep voltammetry PBS phosphate-buffered saline SEM scanning electron microscope TB Tris-HCl buffer PBST phosphate-buffered saline tween
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
Copyright © 2025 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.