The cellulase auxiliary ultrasonic-assisted extraction process of nuciferine from Nelumbo nucifera leaves was optimized by response surface methodology. The extraction content of nuciferine was measured by HPLC, On the basis of single factor experiment, ethanol concentration, liquid-solid ratio, and extraction times were selected as independent variables, and the extraction content of nuciferine was selected as response value, Box-Behnken design was adopted and optimized extraction process in this experiment, the optimum extraction conditions were confirmed. The results showed that the optimum extraction conditions were 10% cellulase, 87% ethanol concentration, 1:26g/mL solid-liquid ratio, 3 times and 25 minutes. The content of nuciferine was 0.343mg/g. The extraction rate of nuciferine was 0.034% after cellulase was added, the extraction rate of nuciferine without cellulase was 0.010%. The extraction process obtained was reasonable, this experiment can provide a more excellent and reliable method for extracting nuciferine from leaves of Nelumbo nucifera.

Many developing countries are facing the difficulty of choosing between economic growth and energy conservation and emission reduction (ECER). China has strengthened the implementation of ECER by setting environmental accountability as the development goal of local governments, hoping to have better governance effects. To evaluate the actual intervention effect of this approach, this paper constructs panel data covering 46 countries from 1995 to 2014 and uses the difference-in-differences (DID) method and the composite control method to quantitatively analyse the policy effect. The results show that China can effectively curb energy consumption and carbon emission intensity per unit of GDP by adding ECER targets to the government’s five-year plan, which has significant effects on ECER. Furthermore, we use an intermediary mechanism to test and identify low-carbon alternatives and an ECER promotion mechanism for technological advancement. The conclusion shows that economic development is compatible with low carbon and energy consumption. Combined with China’s long-term goals for ECER, it can be considered that on the road to achieving carbon peaking and carbon neutrality in the future, the economy and tertiary industry should be rationally developed, the degree of urbanization should receive more attention, and the proportion of thermal power generation should be reduced.

The cellulase auxiliary ultrasonic-assisted extraction process of salidroside from Rhodiola cretinii was optimized by response surface methodology. The extraction content of salidroside was measured by HPLC, On the basis of single factor experiment, extraction time, quantity of cellulase, extraction temperature and ethanol concentrations were selected as independent variables, and the extraction content of salidroside was selected as response value, Box-Behnken design was adopted and optimized extraction process in this experiment, the optimum extraction conditions were confirmed. The results showed that the optimu extraction conditions were as follows: 29 min, 0.18% cellulose, 43°C, 39% ethanol concentration, the extraction content of salidroside was 4.49 mg/g. Through the verified tests, the content of salidroside was 4.39 mg/g after cellulase was added, the content of salidroside without cellulase was 0.48 mg/g. The response surface analysis can predict the experimental results well, and this experiment can provide a more reasonable and effective method for extracting salidroside from R. cretinii.