Design-Expert 7.0 software was used to carry out the response surface test on isoflavones extraction from soybean. The three factors of ethanol mass fraction, pressure and ratio of liquid to solid were investigated and their optimal values were determined to be: 61.50%, 152.00 KPa and 21:1, respectively. Under the optimal conditions, the maximum extraction rate of 3.58 mg/g was obtained, which is about 11% higher than that obtained at atmosphere. The established second multivariate regression equation was proven to be consistent with the experiment and can be used to predict the extraction rate of the isoflavones from soybean under different technical conditions.

The waste wood dust was utilized to prepare the copper oxide loaded activated carbon for the treatment of the printing and dyeing wastewater (acid red GR wastewater). The response surface assisted with Design-Expert 7.0 software was used to optimize the process. The secondary multiple regression models for the color and COD removal rates were established and proven to be significant. The optimum process conditions determined by the software were: copper nitrate (0.5mol/L) 15mL, ratio of liquid to solid 56, activation temperature 690 °C and activation time 2.1h, under which the color and COD removal rates reached 99.8% and 88.34%, respectively.

The extraction of collagen from fish scales with papain under ultrasonic pretreatment was conducted in this paper. The effects of the ultrasonic pretreatment time, the ratio of papain to fish scales, the extraction temperature and the extraction time on the extraction rate of collagen were investigated by single factor experiment and orthogonal experiment, respectively. The results showed that the optimum conditions for collagen extraction from fish scales were: ultrasonic pretreatment time 4min, ratio of papain to fish scales 4%, temperature 60°C and extraction time 5h. Under the optimum conditions the extraction rate of collagen reached 90.7%.

The electroless nickel-phosphor deposition on quartz optical fiber surface was carried out by single factor test. The optimum NiSO4·6H2O, NaH2PO2·H2O , C3H6O2 and H3BO3 concentrations, pH value and temperature were determined to be: 0.1331 mol/L, 0.3019 mol/L, C3H6O20.3761 mol/L, H3BO3 0.4520 mol/L, 4.5 and 84°C, respectively. Scanning electron microscope, stereomicroscope and thermal shock rest were applied to measure the morphology and strength of adhesion of the deposited Ni-P coating. The result showed that a desired Ni-P coating with good smoothness and adhesion was prepared under the optimal conditions. The thermodynamic models were established and proven by the current study.