Fusarium oxysporum causes vascular wilt in more than 100 plant species, resulting in massive economic losses. A deep understanding of the mechanisms of pathogenicity and symptom induction by this fungus is necessary to control crop wilt. The YjeF protein has been proven to function in cellular metabolism damage-repair in Escherichia coli and to play an important role in Edc3 (enhancer of the mRNA decapping 3) function in Candida albicans, but no studies have been reported on related functions in plant pathogenic fungi. In this work, we report how the FomYjeF gene in F. oxysporum f. sp. momordicae contributes to conidia production and virulence. The deletion of the FomYjeF gene displayed a highly improved capacity for macroconidia production, and it was shown to be involved in carbendazim’s associated stress pathway. Meanwhile, this gene caused a significant increase in virulence in bitter gourd plants with a higher disease severity index and enhanced the accumulation of glutathione peroxidase and the ability to degrade hydrogen peroxide in F. oxysporum. These findings reveal that FomYjeF affects virulence by influencing the amount of spore formation and the ROS (reactive oxygen species) pathway of F. oxysporum f. sp. momordicae. Taken together, our study shows that the FomYjeF gene affects sporulation, mycelial growth, pathogenicity, and ROS accumulation in F. oxysporum. The results of this study provide a novel insight into the function of FomYjeF participation in the pathogenicity of F. oxysporum f. sp. momordicae.

Phaeobotryon rhois is an important pathogenic fungus that causes dieback and canker disease of woody hosts. Here, a novel mycovirus, tentatively named Phaeobotryon rhois victorivirus 1 (PrVV1), was identi ed from P.rhois strain sx8-4. The PrVV1 has a double-stranded RNA (dsRNA) genome of 5,224 nucleotides long and it contains two open reading frames (ORF1 and ORF2), which overlap at a AUGA sequence. ORF1 encodes a polypeptide of 786 amino acids (aa) with a conserved a coat protein (CP) domain, while ORF2, encodes a large polypeptide of 826 aa with a conserved RNA-dependent RNA polymerase (RdRp) domain. Our analysis of the genomic structure, homology searches, and phylogeny indicated that PrVV1 is a novel member of the genus Victorivirus in the family Totiviridae. This is the rst study to report the complete genome sequence of a victorivirus that infects P.rhois.

Phaeobotryon rhois is an important pathogenic fungus that causes dieback and canker disease of woody hosts. Here, a novel mycovirus, tentatively named Phaeobotryon rhois victorivirus 1 (PrVV1), was identified from P.rhois strain sx8-4. The PrVV1 has a double-stranded RNA (dsRNA) genome of 5,224 nucleotides long and it contains two open reading frames (ORF1 and ORF2), which overlap at a AUGA sequence. ORF1 encodes a polypeptide of 786 amino acids (aa) with a conserved a coat protein (CP) domain, while ORF2, encodes a large polypeptide of 826 aa with a conserved RNA-dependent RNA polymerase (RdRp) domain. Our analysis of the genomic structure, homology searches, and phylogeny indicated that PrVV1 is a novel member of the genus Victorivirus in the family Totiviridae. This is the first study to report the complete genome sequence of a victorivirus that infects P.rhois.

Fusarium pseudograminearum is a pathogenic fungus causeing crown rot. Only two mycovirus have been reported in F. pseudograminearum thus far. Here, we report a fungal virus (mycovirus), Fusarium pseudograminearum fusarivirus 1 (FpFV1), isolated from the F. pseudograminearum strain ZZ-1. FpFV1 has a single-stranded positive-sense RNA (+ ssRNA) genome of 6622 nucleotides containing four open reading frames (ORFs). ORF1 encodes for a large 1,550 amino acids (aa) polypeptide with a conserved RNA-dependent RNA polymerase (RdRp) and two helicase domains. The ORF2, ORF3 and ORF4 have overlapping regions and encode putative proteins of 154 aa, 53aa and 429aa, respectively. All three ORFs have unknown functions. Based on the genomic structure, homology searches, and phylogenetic analysis, FpFV1 could be a new member of the proposed “Fusariviridae”. This is the first study to report a fusarivirus that infects F. pseudograminearum.