Background: The transcriptional regulator YAP is frequently overexpressed in human cancers, such as breast and pancreatic cancers, plays an important role in tumorigenesis and can regulate many factors affecting cancer progression. These observations encouraged us to investigate the effect of YAP expression on bladder cancer. Methods: The changes in multiple cellular functions associated with tumor progression including cell proliferation, cell migration, cell cycle, and cell apoptosis were assessed after YAP knockdown/overexpression in bladder cancer cell lines. Additionally, Western blot was developed to verify the change of proteins caused by YAP knockdown/overexpression. Results: YAP had relatively higher expression in bladder cancer tissues than in normal tissues. The proliferation and migration of bladder cancer cells were inhibited by YAP knockdown but were promoted by its overexpression. This promoting effect was accompanied by the increased activity of MAPK/ERK pathway. Conclusion: Our data established that YAP is an oncogene involved in bladder cancer and thus can be a potential target for treatment.

To realize the quantitative classification and comprehensive evaluation of the tight oil reservoir in the 4th of Quantou Formation in Songliao Basin, the pore structure of tight oil reservoir was quantitatively analyzed. In this study, experiments based on the constant-speed mercury injection, nuclear magnetic resonance, and micron CT are based on the observation of cast thin sections, and scanning electron microscope is conducted. The result shows that a tight oil reservoir space is mainly composed of the intergranular dissolved pore, residual intergranular pore, and intragranular dissolve pore. There are few intercrystalline pore and fracture at the same time. The average pore radius is 1.28~4.58 μm, and the average throat radius is 0.52~1.27 μm. Hence, there is a typical micro-nano throat combination with micropores. The pore throat composition and pore structure of reservoirs differ in reservoir with different physical properties. The reservoir quality depends on pore structure especially the throat radius. And the microthroat is a critical factor for permeability. Based on Q-cluster analysis, tight oil reservoirs are classified into three types. The analysis was based on the data such as max throat radius, average throat radius, displacement pressure, median pressure, and permeability. Finally, the reservoirs in the study area are mainly II and I types.

To analyze the application value of computed tomography (CT) based on a three-dimensional reconstruction algorithm in perioperative nursing research and prognosis analysis of non-muscle-invasive bladder cancer (NMIBC), a retrospective study was performed on 124 patients with NMIBC who underwent surgical treatment in the hospital. All patients underwent CT examination based on the three-dimensional reconstruction algorithm before surgery, and transurethral resection of the bladder tumor was performed. The patients receiving conventional care were classified as the control group, and those receiving comprehensive care were classified as the case group, and the recovery status and recurrence of the two groups were compared. The results showed that the accuracy, specificity, and sensitivity of CT imaging information based on the three-dimensional reconstruction algorithm for NMIBC patients were 89.38, 93.77, and 84.39, respectively. The incidence of bladder spasm (9.68%), bladder flushing time (1.56 d), and retention of drainage tube time (2.68 d) in the case group were obviously lower compared with the control group (30.65%, 2.32 d, and 5.19 d) ( P  < 0.05). Serum BLCA-1 (3.72 ng/mL) and CYFRA21-1 (5.68 μg/mL) in the case group were significantly lower than those in the control group, with a statistically considerable difference ( P  < 0.05). Compared with the control group, the scores of role function (89.82 points), emotional function (84.76 points), somatic function (79.23 points), and social function (73.93 points) in the case group were observably higher ( P  < 0.05). In addition, one year after the operation, CT examination showed that the recurrence rate in the case group (6.45%) was significantly lower than that in the control group (22.58%) ( P  < 0.05). Therefore, CT detection based on the three-dimensional reconstruction algorithm was particularly important for preoperative diagnosis, prognosis, and recurrence monitoring of NMIBC patients. It could provide great clinical value for the diagnosis and prognosis monitoring of NMIBC.