Dissolved organic matter (DOM) plays a very important role in the dynamics of different biogeochemical processes in the global marine carbon cycle. Seasonal variations of DOM were investigated using dissolved organic carbon (DOC), absorption spectroscopy, and fluorescence excitation emission matrix-parallel factor analysis (EEM-PARAFAC) in Niushan Island in the East China Sea, off southeast Zhejiang, in winter, spring, and summer 2017. A strong positive correlation between temperature and salinity was observed in winter, whereas negative correlations were obtained in spring and summer. The results suggest that the water in the study area originates from the Changjiang River water coinciding with the direction of winds in winter. On the other hand, the Taiwan Strait water flows northward on the eastern part of the study area in summer and spring. The DOC concentration showed significant seasonal variations and was much lower in summer than in winter, but largely independent of the temperature, degree of absorption and intensity of the fluorescence component. Three tryptophan-like components (C1, C2 and C4) and one humic-like component were identified by the PARAFAC model in winter, while two tryptophan-like components (C1 and C2) and two humic-like components were illustrated in summer. The seasonal variations of DOC concentrations and intensities of the fluorescence components in the study area from winter to summer suggest that the waters in this area were influenced mainly by mixing water of the Changjiang River and the Taiwan Strait. A strongly linear relationship was found between the nutrient and the fluorescence intensities of different components in winter, reflecting the profound impacts of the dynamics of DOM on nutrient cycling.