No abstract

A high fat diet significantly impacted gut microbiota composition and lipid metabolism in human flora-associated mice, which were largely ameliorated by tea polyphenol (TP). Therefore, TPs may be effectively used in controlling or treating obesity, hyperlipidemia and other related metabolic diseases.

Rapid urbanization is resulting in large-scale land-use conversion and the impact on ecosystem services value is a key issue for sustainability-particularly in China-a global urbanization hotspot. Impacts of land-use change on ecosystem services value in China have been varied, ranging from strong decreases to modest increases, suggesting that bespoke analyses are required to support local and regional planning for sustainability. We quantified land-use change and valued the impact on ecosystem services from 1990 -2010 in the rapidly urbanizing Guangzhou-Foshan Metropolitan Area, southern China. We quantified land-use change from Landsat satellite imagery and calculated the value of ecosystem services using the well-established unit-value transfer method. Over one quarter of the study area changed land-use between 1990 and 2010 with Built-up, Orchard, and Waterbody land-uses expanding, and Cropland and Forestland contracting. However, the net decline in ecosystem services value was only 4.4% (US$201.5 million). This modest overall decline masked more complex and extreme dynamics in the value of individual ecosystem services. Substantial declines in the value of gas regulation (US$115.4 million, -28%) soil formation and retention (US$90.5 million, -16%), and climate regulation (US$44.6 million, -8%), while waste treatment (US$68.5 million, +10%) and recreation and culture (US$45.7 million, +12%) increased in value. Decision analysis is required to support land-use planning to ensure the sustainability of ecosystem services for the wellbeing of the people of Guangzhou-Foshan and other rapidly urbanizing areas in China and globally.

A loop-mediated isothermal amplification (LAMP) method for rapid detection of the food-borne L. monocytogenes strains had been developed and evaluated in this study. The optimal reaction condition was 65 °C for 45 min, with the detection limit as 1 pg DNA/tube and 100 CFU/reaction. Application of the established LAMP assay was performed on 182 food-borne L. monocytogenes strains using a rapid procedure and easy result confirmation, with the sensitivity of LAMP versus PCR assays as 96.7% (176/182) and 91.2% (166/182), respectively; with 100% specificity, positive predictive value (PPV) and negative predictive value (NPV) for both assays.