For improving the system performance of mobile Internet, how to provide the Quality of Experience (QoE) guarantee is an important factor. First, based on artificial neural network and adaptive cross-layer perceptron, we studied the cloudassisted QoE guarantee mechanism. Then, according to the power, we divided the distance and perceptron layers of mobile Internet and cloud into three levels. We showed the state information definition of the mobile node on the basis of the adaptive adjustment perceptron layers. Thirdly, the perceptron network topology would be updated according to the customer service, which would be updated based on the perceptron learning rule for improving the training practice efficiency. The above scheme would guarantee the QoE effectively. The experimental results show that the proposed QoE guarantee mechanism has obvious advantages in terms of throughput, efficiency, and reliability.