Freeway bottleneck identification is an essential component in the process of deploying mitigation strategies to reduce congestion at freeway bottlenecks. Most previous studies on bottleneck identification focus on recurrent bottlenecks, and limited work has been conducted to identify the locations of non-recurrent bottlenecks. Therefore, in this study, we propose a new travel time reliability (TTR) measurement and develop a freeway bottleneck identification method based on this measurement, which can identify with high probability not only recurrent bottlenecks but also the locations of non-recurrent bottlenecks. The TTR measurement is developed based on statistical distance between travel time distributions. Three statistical distance measurements, Jensen–Shannon divergence, Wasserstein distance, and Hellinger distance, are applied in the TTR measurement. The bottleneck identification method is evaluated in a case study on I-15 freeway corridor in Salt Lake City, Utah. The three statistical distance measurements show good consistency in ranking locations by the impacts of recurrent and non-recurrent congestion, especially for extreme cases with very high or low variation between travel time distributions. The recurrent bottlenecks identified in this study show their clustering characteristics, which is similar to the generating and dismissing process of recurrent congestion. The locations with high probability of non-recurrent bottlenecks scatter both spatially and temporally, which agrees with the random characteristic of non-recurrent congestion.