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follicles following an administration of diazinon.Materials and Methods: A total of 30 adult female Wistar rats were divided into fivegroups: a control group (without any intervention), sham group (received only pureolive oil, as solvent), experimental group I (DZN+olive oil, 60 mg/kg), experimentalgroup II (vitamin E, 200 mg/kg), and experimental group III (DZN: 60 mg/kg+vitaminE: 200 mg/kg). All drugs were injected intraperitoneally, except vitamin E which wasadministrated by gavage. The animals were scarified after two weeks and left ovary wasused to measure proliferation of ovarian follicles. Tissues were analyzed by the PCNAtechnique and viewed with an optical microscope for evaluating cells proliferation.Results: The result of the present study revealed that the number of proliferative cellsin the experimental group I decreased significantly in contrast to the control groupin secondary and Graffian follicles (p< 0.001). The administration of vitamin E plusDZN significantly increased proliferative cells compared to the DZN group (p< 0.001).Primordial follicles showed that all study groups were lacking PCNA positive cells,which means no expression of PCNA in these follicles. The results of this study showedthat primary follicles in all study groups had a few and scattered PCNA positive cellswith no significant difference between the groups (p> 0.05).Conclusion: Results showed that DZN reduced proliferation in secondary and Graffianfollicles and vitamin E increased it. The results of this study suggested that vitamin Eby its antioxidant activity was able to improve the DZN-induced ovarian toxicity.

Background: Diazinon (DZN) is an organophosphate insecticide widely utilized in agriculture all over the world and causes many negative effects on plants and animal species, especially on human. The aim of present study was to evaluate the effects of DZN on apoptosis of ovarian follicles in adult rats and to assess the protective role of vit E. Methods: Thirty adult female Wistar rats were divided into five groups: control group (without any intervention), sham group (received only pure olive oil, as solvent), experimental group 1 (DZN+olive oil, 60 mg/kg), experimental group 2 (vit E, 200 mg/kg), and experimental group 3 (DZN+vit E, the same dosage). All drugs were injected intraperitoneally, except vit E administrated by gavage. The animals were scarified after two weeks and left ovary was used to measure apoptosis of ovarian follicles. Results: The number apoptotic cells experimental group 1 increased significantly in contrast control group in secondary and graffian follicles (P< 0.001). Administration vit E plus DZN, significantly reduced apoptotic cells compared to DZN group (P< 0.001). Conclusion: DZN-induced apoptosis in secondary and graffian follicles and vit E inhibited apoptosis induced by DZN. Vit E might have a protective effect on DZN-induced ovarian toxicity.

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