Acute cholecystitis is a common inflammatory condition of the gallbladder caused most commonly byEscherichia coli,Enterococcus, andKlebsiellaorganisms. Streptococcus bovisis a Gram-positive, catalase-negative, anaerobic coccus found as a commensal inhabitant of the digestive system in 16% of healthy people. We report a rare case of acute cholecystitis caused byS bovisand discuss its implications with regard to the two knownS bovisbiotypes (I & II) both of which are associated with a number of other gastrointestinal diseases.