Present studies were conducted on evaluation of soma-clonal variation of CP67-1026 mutant through morphological procedures with the aims at discussing how soma-clonal variations can be analyzed through morphological approaches in gaining knowledge about the underlined regulatory controlled mechanisms in plants. In vitro experiments were conducted in the Laboratory of Nuclear Institute Atomic Energy (NIA), Tandojam during the year of 2021. The results so far achieved opened further window for deep analysis with reference to functional analysis of soma-clonal variated plants with different approaches. Results on the performance for soma-clonal variation in sugarcane variety NIA-2010 and their soma-clones revealed the best performance of varieties, which showed that the plant height (302.33cm) was observed in SC-3 and the lowest performance was examined in SC-2 that was approximately (154.33cm). The consequences of varieties and diverse parameters indicated that the highest number of internodes plant-1 were recorded (38) in SC-3 and lowest number of internodes plant-1 were accomplished (23) in SC-1. The output of different features indicated that highest number of tillers plant-1 was observed (8.02) in SC-3, the lowest number of tillers plant-1 (4.33) were noted in SC-4 and 8 as well. The consequences of soma-clones indicated that maximum weight stool-1 (kg) was observed (9.67kg) in SC-3 and minimum weight stool-1 (kg) was showed (5.83) in SC-1. The output of different physiological features indicated that highest values were remarkably noticed of brix (%) (19.83%) in SC-13, sucrose (%) (13.4%) in SC-3, fiber (%) (18.92g) in SC-3 whereas the lowest fiber (%) (16.58g) were seen in SC-11. The results of various agronomic parameters such as germination % observed (82.01%) in SC-6, commercial cane sugar percentage (CCS %) showed (9.1%) in SC-9, sugar recovery (%) (8.56%) in SC-8, purity (%) (85.27%) in SC-8, cane yield (tonnes per hectare) (96.67 tonnes per hectare) in SC-3, sugar yield (tonnes per hectare) (7.95 tonnes per hectare) in SC-3 for the assessment of morphological constitutions of NIA-2010 genotype and their soma-clone
The present study aimed to evaluate the initiation, proliferation potential, and mass clonal production ability of a micropropagation system for banana through tissue culture. A total of 60 explants were cultured on basal media supplemented with various concentrations of BAP and NAA. Banana plants regenerated on MS basal medium (control) without the addition of BAP + NAA showed a significantly (P < 0.05) lower survival rate with no signs of shoots up to the end of the experimental period. The results further revealed that the performance in MSS-XI medium was almost 89%, followed by MSS-IX and MSS-X media, both of which showed performance up to 88%. In contrast, the performance in the MSS-XVI medium was less than 60%, at the less duration of time and highly shoot induction detected at MSS-XIII medium. The maximum number of shoots (4.9) was observed in the medium supplemented with growth adjuster MSS-XI, followed by the MSS-XII medium (4.5). Surprisingly, the best performance was observed for the MSR-VII medium approximately 16 days after initiation, while the lowest performance was observed with MSR-XI (approximately 31 days). The maximum rooting percentage (98%) was observed in the MSR-V to MSR-VIII media (98%), while the minimum rooting percentage was observed in MSR-XI (approximately 45%).
The present study was conducted to determine the regeneration potential and producing mass-clonal production of wheat through embryogenesis. A total of 60 selected wheat seeds were surface sterilized and inoculated on different culture bottles under controlled condition. The seeds were emerged in MS basal media and later supplemented with different concentrations of BAP (Benzyl-amino purine) and IAA (Indole-acetic acid). The Observation on servile rate, regeneration capability, length of leaves, length regenerated seedlings, length and number roots were observed. Resultsfurther expressed that the media MS-MIII showed maximum length of leaves and roots of seedlings followed by cultured on media supplemented with 10µg/L or 20µg/L or 30µg/L each of IAA and BAP. Though, seedlings obtained under the concentration of 40µg/L of IAA and BAP were relatively similar and showed significantly moderate result followed by cultured on the medium supplemented with 30µg/L. furthermore, the rooted plants were then transplanted into the substrate and acclimatized in the laboratory greenhouse where humid cavity available. The acclimatization in the humid cavity showed optimistic effect on the number of survived plants.
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