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(2016) A study of the kinetics and the effect of trace elements on mixed anaerobic fermentative biogas production by ternary quadratic general rotary unitized design, Biotechnology & Biotechnological Equipment, 30:1, 90-99, DOI: 10.1080/13102818.2015 , and the maximum CH 4 yield was 241.6 mL/g volatile solids (VS), according to a regression equation. The non-dissolved organic carbon hydrolytic process showed a good fit with the first-order kinetic model. The maximum value of CH 4 was 312.87 mL/g VS. Compared to the control, the bioconversion efficiencies of CH 4 and CO 2 production increased by 36.76% and 74.50%, respectively, at the optimal trace element combination. The obtained results provide new knowledge for improvements in the efficiency of anaerobic fermentation biogas production.