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How to achieve the sustainable and coordinated development of the ecological environment, the economy and tourism has already received much attention. In this paper, a comprehensive evaluation index system of the ecological environment, the economy and tourism is established, and the coupling degrees and coordination degrees of the three subsystems of 31 provinces in China from 2003 to 2017 are calculated. The results show that the average coupling degree and average coordination degree have fluctuating upward trends during the period of 2003-2017. According to the spatial distribution of the coupling degrees and coordination degrees, the coastal provinces and Chongqing, with their high ecological environment pressure and good economic development, have low coupling and extremely high coordination levels. The vast central and western provinces with good ecological environment protection and economic backwardness have high coupling and low coordination development level. From the perspective of coordinated development, only seven of the western provinces and three middle-eastern provinces possess basic coordinated development of the ecological environment, the economy and tourism. The remaining 21 provinces are over-utilizing or sacrificing their ecological environments, among which eleven eastern provinces have an advanced economy or advanced tourism and five southwestern provinces with high tourism resource endowments have an advanced tourism.

Abstract:A planar amperometric glucose microsensor based on glucose oxidase immobilized by chitosan film on Prussian Blue layer has been developed. The experimental results show that the optimum detection potential is 50 mV (versus Ag/AgCl) and the optimum pH is 6.5. Under the selective conditions the sensor exhibits excellent sensitivity of 98 nA/M and a linear range of 0.1-6.0 mM. The apparent Michanelis-Menten constant of the sensor is 21 mM. The response time is less than 60 seconds. No apparent change in the response to glucose was observed during one month. Foremost, the interference of ascorbic and uric acids can be avoided due to selective permeability of chitosan film and electrocatalysis of PB layer to H 2 O 2 . The sensor has been applied to detect glucose in human blood serum.

Chitosan/Prussian blue (PB) based biosensors, including a glucose sensor, a glutamate sensor and a galactose sensor have been developed. The biosensors exhibit excellent performance; in particular, the interference of ascorbic and uric acids can be avoided due to selective permeability of chitosan film and electro-catalysis of the PB layer to H 2 O 2 . The biosensors have been applied to detect glucose, galactose and glutamate in human blood serum and fermented solution.