Antiaging protein Klotho exhibits impressive properties of anti-inflammation, however is declined early after intervertebral disc injury, making Klotho restoration an attractive strategy of treating intervertebral disc inflammatory disorders. Here, we have found that Klotho is enriched in nucleus pulposus (NP) cells and Klotho overexpression attenuates H2O2-induced acute inflammation essentially via suppressing Toll-like receptor 4 (TLR4). The proinflammatory NF-κB signaling and cytokine expressions paralleled with Klotho repression and TLR4 elevation in both NP cells (H2O2 treatment) and rat intervertebral disc (needle puncture treatment). Overexpression of TLR4 downregulated expression of Klotho, whereas interfering TLR4 expression diminished the inhibitory effects of H2O2 on Klotho in NP cells. Consistently, Klotho knockdown by RNA interferences largely diminished the anti-inflammatory and intervertebral disc protective effects in an Intervertebral Disc Degeneration (IDD) model. Thus, our study indicates that TLR4-NF-κB signaling and Klotho form a negative-feedback loop in NP cells. Also, we demonstrate that the expression of Klotho is regulated by the balance between upregulation and downregulation of TLR4-NF-κB signaling.

This paper aimed to explore the application values of computed tomography (CT) imaging in the treatment of patients with severe traumatic brain injury (STBI), to provide help in the treatment of STBI. In this study, 86 patients with STBI were selected as the research objects and examined by CT based on a self-adaptive particle swarm algorithm (APSO). Besides, patients were treated with hyperbaric oxygen, high-dose hormone shock, and naloxone hydrochloride. The results showed that there was a locally low-density brain contusion and laceration injury area, spot-like high-density hemorrhage, and subarachnoid hemorrhage in the images of CT examination. The ventricles of diffuse brain injury were compressed and reduced, and the white matter indicated that the ventricles and cisterns became smaller. Asymmetric hemorrhage and subarachnoid hemorrhage were scattered at the white matter junction. In short, subarachnoid hemorrhage, spot-like high-density hemorrhage at the injury site, and diminished ventricles were typical CT imaging manifestations of STBI. Naloxone hydrochloride method could effectively alleviate the matrix metalloproteinase-9 (MMP-9) (23.47 ± 3.45) and S100 calcium-binding protein B (S100B) (0.16 ± 0.06) of patients, which had reliable guiding significance for the later CT examination clinically.