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<p>After a long period of water flooding development, oilfields will enter the production stage of "high water cut, high recovery degree, and low oil recovery rate". On the one hand, due to the displacement effect of the water injection, some oil layers already reached the water flooding limit. On the other hand, due to the effect of reservoir heterogeneity, dominant seepage channels, and imperfect injection-production well pattern, some oil layers are enriched with a large amount of remaining oil. Unbalanced production of reservoirs and difficulty in development and adjustment are common problems in mature oilfields. Mature multi-layer oilfields generally develop many sets of oil-bearing layers vertically. After a long water injection period, the water-flood law and the remaining oil distribution are complex, and the production of different well patterns or strata varies greatly. Through strata and well pattern reorganization, combined with the evaluation results of water flooding adjustment potential, some reservoir engineers and researchers established a stereoscopic development adjustment mode for enhanced oil recovery in mature multi-layer oilfields. This paper summarizes the main technologies of stereoscopic development adjustment mode for enhanced oil recovery in mature multi-layer oilfields. The main technologies of stereoscopic development adjustment mode include research on the remaining oil distribution, evaluation of water flooding adjustment potential, selection of tertiary oil recovery methods, reorganization of strata and well pattern, and optimization of timing from water flooding to tertiary oil recovery, etc. For strata with low water flooding adjustment potential, the tertiary oil recovery well pattern is reorganized and tertiary oil recovery is adopted to improve oil recovery. For strata with large water flooding adjustment potential, the water drive well pattern is reorganized and water flooding development is used to excavate the remaining oil. As for strata with general water flooding adjustment potential, the tertiary oil recovery well pattern is reorganized and water flooding development is used to excavate the remaining oil first, and then transfer to tertiary oil recovery at the proper time. The stereoscopic development adjustment mode is applied to test block K of Q reservoir which is a mature multi-layer oilfield. After stereoscopic development adjustment, the development effect of test block K meliorates. It is estimated that the EOR will be increased by more than 8% after stereoscopic development adjustment in test block K.</p>