This study aimed to determine the effect of fermented kelor leaves (Moringa oleifera) flour as additive in animal feed to increase the weight of boiler carcass. The samples used were 28 DOC strain Cobb CP-707 aged 1 day. This study was conducted using one way pattern complete randomized design with 4 treatments and 7 replications. Control group (P0) was fed with commercial feed; P1 was given 5% fermented kelor leaves flour; P2 was given 7,5% fermented kelor leaves flour; and P3 was given 10% concentration of fermented kelor leaves flour. Feed replacement was conducted on 08.00 am and 5.00 pm everyday, and water was given ad libitum. Chemical content of flour before and after fermented was analyzed at Research Centre of Biotechnology and Biological Resources, IPB. Body weight measurement was carried out at Veterinary Public Health Laboratorium. Data were analyzed using ANOVA with SPSS 16.0. Mean value (±SD) of carcass weight for P0, P1, P2, and P3 were 938,71±180,83, 1011,86±101,64, 1010,71±126,69, and 907,43±190,35, respectively. This study showed that adddition of fermented kelor leaves flour for 21 days did not affect (P0,05) the weight of broilers carcass.
This study aimed to compare the progesterone concentrations in Waringin sheep estrus cycle which induced by PGF2α or the combination of PGF2α and GnRH. This study used six Waringin sheep those were divided into twice groups, K1 and K2. K1(n=3) were injected by 7.5 mg PGF2α intramuscularly for 10 days, while K2 (n = 3) were injected by PGF2α and GnRH. On the first day K2 were injected by 7.5 mg PGF2α, then followed by injection 50 µg of GnRH on day 8th and re-injected by 7.5 mg PGF2α on day 15th. On day 18th, sample was re-injected using 50 µg GnRH. Waringin sheep those showed estrus symptoms were detected visually and with signs showed by sheep stud. Blood samples were taken on the 7th, 14th, and 21st day after the peak heat. The measurement of progesterone concentration was conducted by enzyme linked immunosorbent assay (ELISA). The results showed those the concentrations of progesterone on day 7th, 14th, and 21st on K1 vs K2 were 1.324±1.079, 7.607±8.922, and 5.220±1.653 vs 4.705±3.369, 4.184±5.512, and 1.797±0.898 ng/mL (P>0,05), respectively. In conclusion, the concentration of progesterone of Waringin sheep after inducing with PGF2α or combination PGF2α and GnRH at different cycle periode did not show differences. Keywords: Estrus Cycle, GnRH, PGF2α, Progesterone, Waringin Sheep
The aim of this research was to find out the effect of water temperature increase on leucocyte count of nile tilapia. The blood sample from 9 tilapia with weigh of 40-50 g were used in this study. The research was conducted using completely randomized design (CRD) with 3 treatments and 3 replications. Treatment P1 as a control with aquarium temperature was maintained at 29±1 C. Water temperature in treatment P2 and P3 was maintained at 32±1 C and 35±1 C respectively. The aquarium size in each treatment group was 80x60x40 cm with height of water 30 cm. The treatment was done for 15 days and on day 16th, the blood were collected from caudal vein. Data were analyzed with analysis of variance (Anova) and followed with Duncan's multiple range test. The result showed that total leucocyte count (x 105/mm3) of nile tilapia in group P1, P2, and P3 were 3.08±0.88, 1.70±0.52, and 1.69±0.20 respectively. lts showed that an increase in temperature significantly affect (P<0.05) the leucocyte count of nile tilapia. The conclusion of this research is the increase in water temperature in aquarium decrease the leucocyte count of nile tilapia.
The aim of this research was to evaluate the effect of chayote (Sechium edule) leaves infusion on hemoglobin and hematocrit level of anemic rat (Rattus norvegicus). Twelve anemic hemorrhagic male rats strain Wistar with body weight ranging from 175-200 g were used as sample in this research. Rats were divided randomly into 3 treatment groups, each group consist of 4 rats. The control group (P0) was not given chayote leaves infusion. Group P1 and P2 were given chayote leaves infusion with the concentration of 25% and 50% respectively, with dose of 1 ml per 200 gram body weight. Rats were fed with standard commercial food (Turbo feed) and aquadest ad libitum. The rats were treated anemic by taking 20% blood from total blood volume prior to administration of chayote laeves infusion. Chayote leaves infusion was given for 10 days consecutively. Blood sampling was carried out on day 0, 5, and 10 via caudal vein. Data were analyzed using anlysis of variance (ANOVA) Split-Plot pattern. The result showed that chayote leaves infusion can increase level of hemoglobin and hematocrit in experimental rats. This research conclude that 25% concentration of chayote leaves infusion is more effective than 50% concentration.Key words: anemia, chayote leaves, hemoglobin, hematocrit
This research aimed to determine glucose levels of beagle which is maintained by Sports Association of Pig Hunting (PORBI) in Kenagarian Mungo Sub-district Luak District Lima Puluh Kota, West Sumatra. Blood samples were collected from 20 beagles. Blood sampling was performed during fasting time and two hours postprandial. Data were analyzed with analysis of Chisquare (x2). The results showed that mean glucose level in the fasting state beagle was 63.45±11.84 mg /dl and two hours postprandial was 93.45±12.52 mg/dl. The test results showed statistically significant differences (P<0.05) postprandial, hunting frequency and multivitamin injection on blood glucose levels. The test results showed no statistically significant difference (P>0.05) between the frequency of feeding and supplementary feeding on blood glucose levels. Conclusion average blood glucose levels beagle still in the normal range. There is a difference in blood glucose between two hours postprandial with a fasting blood glucose levels and the difference between the hunting frequency and giving multivitamins injection on blood glucose levels and there was no difference on blood glucose levels between the frequency feeding, and supplemental feeding.Key words: beagle, glucose levels, hunting, multivitamins
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