Introduction: Aloe vera has anti-bacterial ability against Gram-positive and Gram-negative bacteria; however, anti-bacterial study, especially in multidrug resistant strain of Mycobacterium tuberculosis, has not been evaluated yet. This study aims to determine the potential of Aloe vera as an antituberculosis against drug-resistant strains of tuberculosis. Method: Stages of research include the production of ethanol extract of Aloe vera, then testing the phytochemicals (identification of alkaloids, steroids/triterpenoids, anthraquinone, flavonoids, saponins, tannins) and chemical content testing by thin layer chromatography (TLC). The anti-bacterial test for Mycobacterium tuberculosis was performed on the Lowenstein Jensen media. Results: Test results showed the qualitative identification, and TLC contains alkaloids, steroids/triterpenoids, anthraquinone, flavonoids, saponins, and tannins. Antioxidant activity against DPPH radical shows an IC50 value of 6927.133 ppm. Test of anti-bacterial activity to Mycobacterium Tuberculosis H37Rv and MDR TB strains HE (resistant to INH and Ethambutol), and SR (resistant to streptomycin and Rifampicin) showed inhibition ranging concentration of 50 mg/mL in all extracts as well as to test the sensitivity, a sensitive start to a concentration of 50 mg/mL in all extracts. Conclusions: Aloe vera contains alkaloids, steroids/triterpenoids, anthraquinone, flavonoids, saponins, and tannins that act as antioxidants and antituberculosis against strains of Mycobacterium tuberculosis that are not resistant and resistant to anti-tuberculosis drugs.

Objective: To Assess the Level of Knowledge of Breastfeeding Mothers About the Lactation Amenorrhea Method (LAM).Methods: Description research with a population of 75 respondents and sampling of all breastfeeding mothers. The data analysis used is univariate analysis.Results: Most of the respondents were aged between 26-35 years (73.3%), more than half of them had their last education at the upper secondary level (50.7%), most of the respondents were unemployed (72%), the most parity was primigravida (68%), all respondents had been exposed to information about LAM (100%) which was mostly sourced from electronic media (92%) and most respondents had sufficient knowledge about LAM (40%) .Conclusion: Most of the respondents have sufficient knowledge. Therefore, the role of health workers is needed to further intensify information about lactational amenorrhea contraceptive methods so that they can use them correctly.