Mulberry is considered as an important plant in traditional Chinese medicine, due to its various compounds, including phenols and flavonoids. These flavonoids have antioxidant activities so that can be a potential anticancer candidate. The aims of this study were to determining antioxidant activity, phenolic content, and potential anticancer activity in Mulberry stem extract. The extraction was carried out by maceration using ethanol as the solvent, antioxidant activity test using ABTS (2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) method, phenolic content determination using Folin-Ciocalteu reagents, and anticancer activity test using the MTT (3-(4,5-dimetiltiazol-2-il)-2,5-difenil tetrazolium bromida) method on WiDr cancer cells and Vero cells. The result of total phenolic Mulberry stem extract was 35.9%, the antioxidant activity value was 83.18 µg / mL, the IC50 value for anticancer activity for WiDr cells was 71.24 µg / mL and Vero cells IC50 value was 154.241 µg / mL. It could be concluded that the Mulberry stem ethanol extract had strong antioxidant activity and had the potential anticancer selectively against cancer cells WiDr.

<div class="WordSection1"><p align="center"><strong>Abstrak</strong></p><p align="center"><strong> </strong></p><p>Murbei merupakan salah satu obat yang penting didalam pengobatan tradisional Cina. Murbei mengandung berbagai macam senyawa diantaranya fenol dan flavanoid yang umunya berfungsi sebagai antioksidan alami dan juga memiliki fungsi antikanker. Penelitian ini bertujuan untuk mengetahui kadar fenolik, aktivitas antioksidan dan antikanker ekstrak batang murbei (<em>Morus alba </em>L.). Pembuatan ekstrak dilakukan dengan cara maserasi menggunakan pelarut etanol. Penetapan kadar fenolik menggunakan reagen Folin-Ciocalteu, pengujian aktivitas antioksidan menggunakan metode ABTS dan pengujian aktivitas antikanker menggunakan metode MTT terhadap sel kanker WIDR dan sel vero. Hasil pengujian menunjukkan kadar total fenolik ekstrak batang murbei sebesar 35,9%. Nilai aktivitas antioksidan ekstrak batang murbei adalah  83,18 µg/mL. Aktivitas antikanker pada sel WIDR menunjukkan nilai IC₅₀ 71,24 µg/ml dan pada sel vero memiliki nilai IC₅₀ 154,241 µg/ml. Dapat disimpulkan bahwa ekstrak etanol batang murbei (<em>Morus alba </em>L.) memilki aktivitas antioksidan yang kuat dan berpotensi sebagai antikanker alami yang bekerja selektif terhadap sel kanker.</p><p> </p><p><strong>Kata kunci :</strong> Murbei (<em>Morus alba </em>L.), batang, IC₅₀, antioksidan, antikanker</p><p align="center"><strong> </strong></p><p align="center"><strong><em>A</em></strong><strong><em>ntioxydant and anticancer </em></strong><strong><em>effect </em></strong><strong><em>of murberry (Morus alba </em></strong><strong><em>L</em></strong><strong><em>.) <br /> stem extract in vitro</em></strong></p><p align="center"><strong><em> </em></strong></p><p align="center"><strong><em>Abstract</em></strong></p><p align="center"><strong><em> </em></strong></p><p><em>Mulberry is one of the important medicines in traditional Chinese medicine. Mulberry contains a variety of compounds including phenols and flavonoids which generally function as natural antioxidants and also have anticancer functions. This study aims to determine phenolic levels, antioxidant activity and anticancer of mulberry stem extract (Morus alba L.). Extracts were made by maceration using ethanol as a solvent. Determination of phenolic levels using the Folin-Ciocalteu reagent, testing of antioxidant activity using the ABTS method and testing of anticancer activity using the MTT method against WIDR cancer cells and vero cells. The test results showed total phenolic levels of mulberry stem extract amounted to 35.9%. The antioxidant activity value of mulberry stem extract was 83.18 µg / mL. Anticancer activity in WIDR cells showed IC50 values 71.24 µg / ml and vero cells had IC50 values 154.224 µg / ml. It can be concluded that the ethanol extract of mulberry stem (Morus alba L.) has a strong antioxidant activity and has the potential as a natural anticancer that works selectively against cancer cells.</em></p><p><em> </em></p><p><strong><em>Keywords:</em></strong><em> Mulberry (Morus alba L.),</em><em> </em><em>stem extract</em><em>,</em><em> IC₅₀, antioxidant, anticancer</em></p></div><p align="center"><strong><br clear="all" /></strong></p>