Purpose: The study emphasized on exploring the antecedes of intrapreneurship by testing the career construction theory and perceived personality study from the context of Pakistan’s private sector universities. Design/Methodology/Approach: A Total of 270 male and female academicians working in private sector universities had participated in this cross-sectional research study. The survey questionnaire was adopted from different authentic sources and evaluated for validity and reliability. MPlus software has been used for statistical findings. Findings: It has been estimated statistically that career adaptability overall mediates between the relationship of perceived personality traits and Intrapreneurship. Implications/Originality/Value: It might be established that all the public/private sector universities might follow almost the same policies and laws but there are some differences like payment of salaries / remuneration of the faculty members working in government sector universities and private sector universities of Punjab, Pakistan. The study highlighted such issues of personality directly and indirectly with career adaptability and intrapreneurship.