Improving modern dairy farming is based on the industry's development, which means transforming livestock farming into a competitive industry. The development of its technical and technological support is closely related to its functional ability to realize the genetic potential of biological objects of production with high-quality products, which is the defining criterion for the competitiveness of machinery and technology at the industry’s present stage of development. The purpose of the research was to study milk production technology based on a comprehensive assessment and implementation of modern milking equipment, maximizing animals’ genetic potential and industry efficiency. We proved that applying the ‘DeLaval’ milking parlor under the same feeding and keeping animals ensured the proper completeness of milking and increased cows’ milk yield. During the first, third, and highest lactations, the milk yield was 226 kg (P=0.05), 266 kg (P=0.05), and 214 kg (P=0.05), respectively. After-milking when applying ADM-8A averaged 175 ml, and only 45 ml when applying the ‘DeLaval’ parlor. When using the ‘DeLaval’ milking equipment, the milk production intensity of cows was 1.43 kg/min, and, with ADM-8A, it was 1.24 kg/min, which is 0.19 kg/min (P=0.001), or 15.3% higher.

The article presents the results of studies of erythrocytopoiesis in horses with herpesvirus infection of the first type (rhinopneumonia) and respiratory manifestations of herpesvirus of the second type and their coexistence in animals. In the general pathology of horses, herpesvirus infections of horses occupy a leading place, and diseases of mixed etiology, which occur with atypical manifestations of clinical signs, are of particular concern. The work was performed in 2018 on the basis of the laboratory of the Department of Microbiology, Pharmacology and Epizootology of Polissya National University on breeding horses belonging to the Nahiryan branch of PJSC "Rise-Maximko" Ternopil region. Blood for the study was taken from the jugular vein against Jugulares in the morning before feeding Diagnostic studies to confirm herpesvirus infection were performed in the reaction of delayed hemagglutination, reaction and diffusion deposition - for herpes of the first type and in the reaction and diffusion precipitation to herpesvirus infection of the second type. Evaluation of erythrocyte indices allows you to get an idea of the characteristics of erythrocytes, which is very important in determining the type of anemia. Erythrocyte indices often respond quickly to the treatment of anemia and can be used to assess the effectiveness of therapy. The studies revealed a significant decrease in the number of leukocytes P ≤ 0.05 in animals of all experimental groups. Regarding hematocrit and erythrocyte counts, a significant decrease in P ≤ 0.05 was found in horses affected by EHV-1, and in the case of coexistence of two types of pathogens - a significant decrease in P ≤ 0.01. Erythrocyte index indices for herpesvirus infection of the first type in horses were characterized by a significant P ≤ 0.05 increase in the mean hemoglobin concentration in erythrocytes and a significant P ≤ 0.05 decrease in the mean erythrocyte volume. In herpesvirus infection of the second type, the fluctuations of the indices were not significant. In the combined course of the two types of viruses in horses, a significant increase in P ≤ 0.05 hemoglobin content in one erythrocyte and a significant P ≤ 0.05 increase in the average volume of erythrocytes.

The distribution of herpes virus infections of the first and second type in tribal horses for the last five years has been established. During this period, a 1301 samples of blood serum samples of horses were investigated in the diffusion precipitation process. Out of these, 412 or 31.6% of the studied specimens were positive for the herpes virus infection of the first type, 473 or 36.4% for the second-generation herpes virus infection, and 345 or 26.5% of the animals were co-infected with herpes virus infections of the first and second type, respectively. The determination of the epizootic situation in the regions shows that the highest percentage of infections with herpes virus of the first and second types is established in Ternopil region, and the smallest in Chernivtsi. In breeding reproducers of the Chernivtsi region, the level of infection of EHV 1 and EHV 2 types does not exceed 10% of the population. We conducted a study on the coexistence of herpes virus infections, leptospirosis and helminthiasis in horses. The results of the coprological research provide an opportunity to state that in all regions the strongilidosis of horses are the most common. Also, frequent cases of infection with dictiocaulosis testify to the grazing of horses on compatible pastures with cattle. The study, based on the latent course of leptospirosis, depending on the severity of the degree of damage by strontium glands, suggests that with a consistent course of leptospirosis and strontium glands increases the number of leukocytes, eosinophils, neutrophils and total protein. In horses positive in the RMA for leptospirosis and free from strontium glands, a decrease in the number of erythrocytes, lymphocytes, eosinophils and an increase in the number of leukocytes, neutrophils and total protein is observed. In the horses positive in the RMA and affected by strontium, there is a tendency to increase the number of red blood cells, leukocytes, and eosinophils. However, it was found that with an increase in antibody titers in the RMA 1:100 and the intensity of invasion by strontials of 100 and more eggs in 1 gram of feces, a significant decrease (P < 0.001) of the absolute number of lymphocytes and a significant increase (P < 0.001) of the absolute number of eosinophils and neutrophils. A significant decrease in the absolute number of lymphocytes in horses with latent leptospirosis with antibody titres of 1:100 in the RMA and the presence of 100 and more eggs in 1 gram of feces may indicate the development of the immunodeficiency state in such animals and the desirability of immediate demyelination.

One of the most important directions in the development of modern veterinary medicine is the improvement and implementation of modern methods of diagnosis and prognosis of diseases based on determining the degree of dysfunction and the possibility of correcting the protective functions of the body. for most veterinary clinics, the study of the immunological status of animals is not available, and clinical blood tests make it possible to determine the assessment of the functional state of the immune system by integrated hematological parameters. In the article on the basis of blood formulas that reflect the state of neurohumoral homeostasis and immunological reactivity of the organism, for the first time investigated and established violations of humoral and cellular immune systems, micro- and macrophage systems, reduced nonspecific protection and found relationships between immunity in 23 dogs with leptospirosis, which calculated 19 markers of inflammatory activity, endogenous intoxication, adaptation, nonspecific and immune reactivity. The study was conducted in veterinary clinics in zhytomyr and kyiv, blood for the study was taken from the superficial vein of the forearm v. anterbrachium, medial subcutaneous vein or subcutaneous tibial vein v. saphena. Diagnostic studies to confirm leptospirosis were performed using rapid tests Combo Test IgG Ab/IgM Ab, vetlaboratories and elisa on the basis of a private veterinary laboratory of Llc “Bald” in Kiev. The interpretation of integrated leukocyte blood parameters revealed the development of autoimmune processes with suppression of cellular immunity and the development of endogenous intoxication, which indicates an increase in total (P < 0.01), leukocyte (P < 0.05) and nuclear index (P< 0.001). Suppression of the immune response and increased hypersensitivity of the immediate type based on a decrease in non-specific reactivity by P < 0.001 and inflammation index by P < 0.05. It is proved that integrated hematological parameters of peripheral blood increase the informativeness of the general analysis of blood in dogs with infectious diseases and allow to determine not only the degree of reactivity of the organism, but also to assess the level of endogenous intoxication.

Based on the analysis of literature sources, monitoring results and our own research, it has been established that viral enteritis occupies a leading place in the infectious pathology of dogs and causes significant damage to animal owners. Viral enteritis, most commonly caused by parvovirus, causes severe gastrointestinal disorders and is one of the most common causes of infectious diarrhea in dogs under 6 months of age. Therefore, given the relevance of this issue, the result of our study is to clarify, supplement and summarize data on the pathomorphological features of the intestinal form of parvovirus infection in dogs. Diagnostic studies to confirm parvovirus enteritis were performed using VetExpert CPV Ag or CPV/CCV Ag rapid tests, as well as by ELISA and PCR. Dissection of dead animals was performed in the conventional sequence. For pathomorphological examination, samples were taken from the gastrointestinal tract, fixed in 10 % neutral formalin solution, followed by dehydration in alcohols of increasing concentration and the procedure of organ compaction according to the generally accepted method. Histological sections 6–10 μm thick were obtained using a microtome. The article presents the results of pathological changes in dogs with parvovirus enteritis, namely macroscopically under the serous membrane of the stomach revealed striped hemorrhages, duodeno-gastric reflux, manifested by gray-green color of the mucous membrane (bile accumulation). Histological methods have shown that the blood vessels of the submucosal base of the mucous membrane are clearly dilated, filled with blood. The mucous membrane is swollen, unevenly colored in all parts of the stomach. In the muscular membrane of the stomach wall, the vast majority of cells were in a state of granular dystrophy, and in some animals, necrotic changes in smooth muscle tissue were recorded. Histochemical studies revealed the development of microscopic changes that indicated hypersecretion of mucus (acidic proteins) in the stomach of sick dogs. No specific macroscopic changes in the colon were recorded. Microscopically, a small amount of cellular detritus with mucus fragments, goblet cell hyperplasia, and a reaction of epithelial cells that were in a state of granular, less often, hydropic dystrophy were recorded on the lumen of the colon on the mucosal surface.