The aim of the work was to explore the capabilities of LDF method in studying the human skin blood and lymph microcirculation. Materials and methods. It was assumed that analyzing LDF signals in different frequency ranges of the Doppler shift allows the evaluation of the motion of various scattering particles. Results and discussion. When conducting functional tests, the reverse reaction of the microcirculation index in the low frequency ranges was observed in comparison with the high-frequency ones. Additional differences were obtained in the wavelet analysis of LDF signals. Conclusions. The proposed approach with division into frequency ranges proved useful in the joint study of microhemo- and lymphocirculation.

The purpose of this work is to diagnose microcirculatory disorders in the tissues of psoriatic plaques in patients with psoriasis. Material and methods. Using the method of laser Doppler flowmetry (LDF), local hemodynamic parameters were studied in the place of psoriatic lesion of tissue and in uninvolved skin. Results. In the psoriatic plaque, there is an increase in average perfusion, myogenic and cardiac fluctuations, as well as nutritive blood flow as compared to the area of intact skin. Conclusions. The use of spectral analysis of the LDF signal has great potential for assessing the contribution of each link of the microvascular bed in the study the pathogenesis of psoriasis and evaluating the effectiveness of the therapy.

The work is devoted to review the various methods and approaches currently used within the framework of the concept of optical «biopsy» in the study of blood perfusion and metabolism of human organs tissues. The purpose of the review was to study the features and areas of application of these methods and to assess further application possibilities for intraoperative data acquisition in minimally invasive surgery of hepatopancreatoduodenal organs. Preliminary experimental studies were performed with fluorescence spectroscopy method in pathologies of the common bile duct, gallbladder and liver. The data obtained in the work show the prospects for this method application, and serve as the basis for further research in order to obtain additional diagnostic criteria.