The results of long-term authors studies of the optical and complex-forming properties of more than 30 synthetic low-molecular-weight fluorophores specific for DNA are described. These studies have significantly expand the already existing database of properties of such compounds and clarify ideas about the patterns linking the mentioned properties of fluorophores with their structure, and formulate recommendations on the design of new, effective, DNA‑specific fluorophores. The results of these studies can be used, in particular, in the development of new express methods for diagnosing various diseases biotesting of pro- and antibiotic properties of various products and wastes, etc.
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