Bifidobacteria commonly known as classic probiotics and today they gain huge popularity among supporters of wealth nutrition. These microorganisms colonize the intestines of a newborn baby through the mother's milk and joining him in a stable symbiosis, accompany the person throughout his life, exerting a beneficial effect on his health. These amazing microorganisms have great biotechnological potential and are widely used in medicine, veterinary medicine, food and pharmaceutical industries, agriculture and other fields of national economy. The beneficial effects of bifidobacteria on the host organism are vast and diverse. They are involved in enzymatic processes, perform vital minerals function (synthesis of vitamins B, K, folic and nicotinic acids), improve indices of protein, lipid, and mineral metabolism/ They also reinforce the hydrolysis of proteins, carbohydrates ferment, hydrolyzed fats, dissolve fiber, stimulate peristalsis, promote normal evacuation of the intestinal contents, promote the synthesis of essential aminoacids, better assimilation of calcium salts and vitamin D. Moreover, they have anti-anemic, anti-allergic action, and stimulate the lymphoid apparatus. The article presents a review of important technological and biological properties of bifidobacteria, which determine their biotechnological potential: culture-morphological, physiological, biochemical properties; habitat, taxonomy, nutrient medium, and conditions of incubation, antagonistic, antioxidant properties, acid resistance, probiotic properties, the practical application of bifidobacteria.