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The development of a DNA-vaccine against classical swine fever (CSF) is a perspective direction, because it gives an opportunity to develop a marker vaccine due to use of a part of protective antigen molecule, and to induce effectively both cellular and humoral immune response. In this study a recombinant plasmid, containing the fragment of E2 gene of CFS virus (CSFV) in eukaryotic expression vector, has been developed. It has been demonstrated that the fragment of E2 protein of CSFV is expressed in CHO-K1 cells from the developed recombinant plasmid pTR-BKneo -, and we suggest that the protein possesses the post-translational modifications. The data obtained are in favor of the created model DNA-vaccine able to induce humoral immune response to fragment of E2 protein of CSFV.

The ex pres sion of Mgmt gene was in ves ti gated by West ern blot anal y sis in the spon ta ne ously im mor tal ized mouse cell line G1 and in its sublines G1-OA and G1-T at dif fer ent pas sages of in vi tro cul ti va tion. The highest level of Mgmt ex pres sion has been re vealed in G1-T subline cells and the low est-in G1-OA subline cells. The in crease in the level of DNA re pair en zyme Mgmt was ob served in the cells of mouse cell line G1 as well as in its subline G1-OA at later pas sages of in vi tro cul ti va tion. Since the G1-OA subline is char ac ter ized by the high est fre quen cies of chro mo somal ab er ra tions, micronucleate and multinucleate cells, it is pos si ble to sup pose a role of de fi ciency of DNA re pair en zyme Mgmt in the in crease of the chro mo somal ab er ra tion level in G1-OA subline.