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As a result of stage screening, there were selected promising bacterial strains to create laboratory samples of biological products on their basis to protect winter wheat from the causative agents of fusarium root rot. The purpose of the work is to determine the growth-stimulating and protective effect of laboratory samples of Bacillus subtilis BZR 336g and B. subtilis BZR 517 on winter wheat plants, as well as to determine the antagonistic effect of strains on Fusarium fungi. As a result of the work, there was established a positive effect of laboratory samples on the growth, development and germination of plants in greenhouse conditions and the ability to significantly inhibit the mycelium of phytopathogenic fungi F. graminearun, F. culmorum and Microdochium nivale in laboratory conditions. The study of the samples artificially infected in a climatic chamber showed not only a high protective effect of these laboratory samples, but also made it possible to determine the regimes for their use. The conducted tests prove the viability of new bioagents as environmentally friendly plant-protective products.