Aims:To compare the apical sealing ability of TotalFill BC, AH Plus Jet, and GuttaFlow Bioseal sealers. Materials and Methods: Thirty single rooted mandibular premolars were decoronated and standardized at 15mm. The specimens were chemo-mechanically prepared, and randomized into three experimental groups (n =10) according to the root canal sealer tested: TotalFill BC, AH Plus Jet and, GuttaFlow Bioseal. The specimens were filled using single cone technique. The specimens were decalcified, dehydrated, and cleared. The specimens were analyzed by stereomicroscope, and digital images were captured using stereomicroscope attached camera. The apical dye leakage depth was measured and evaluated using four grade scoring system at 10X magnification. The apical micro leakage data among experimental groups were statistically analyzed by one way analysis of variance (ANOVA) test and Fisher's exact test at 5% significance. Results: Statistically, there was no significant difference in apical micro leakage among the experimental root canal sealers. (P>.05) Conclusions: In this study, no experimented root canal sealer had perfect sealing ability. The experimental root canal sealers were similar in their sealing ability at apical area of root canal.