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Herein, physical evaluation of a mass ionization assist agent prepared using mist deposition method and the visualization of natural occurring poisons in plants were investigated. An -cyano-4-hydroxy-cinnamic acid (CHCA) crystal layers were formed on sliced botanical samples deposited on a glass slide for a mass ionization assist agent of matrix assisted laser desorption/ionization(MALDI) imaging. The formed CHCA crystal layer exhibits a homogeneous surface at nozzle temperatures over 200 ℃, and its chemical stability is confirmed via Raman spectroscopy. -chaconine and -solanine in sample were detected as H + adduct localized around of root in sliced potato sample using MALDI-fourier transform ion cyclotron resonance mass spectrometry and mass spectrometry imaging. Mist deposition method is effective for the mass ionization assist preparation and promising for utilize to visualization of target components in samples.

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