Atractylodes lancea is a medicinal plant grown in Jiangsu Province, China. The diversity of the endophytic bacteria culturable from the leaves of A. lancea and their plant growth鄄promoting potential, including nitrogen fixation, phosphorus solubilization, potassium solubilization and indole acetic acid (IAA) production were investigated. According to morphological characteristics of bacterial colonies, 52 endophytic bacterial strains were obtained and 45 could be subcultured normally in vitro and subjected to amplified ribosomal DNA restriction analysis (ARDRA). Based on the ARDRA fingerprints, the 45 subculturable endophytic bacterial strains could be classified into 14 clusters. The 16S rDNA fragments of representative strains from different clusters were sequenced and deduced by a BLAST search to identify similar strains. The results showed that the isolated endophytic bacteria most likely belong to Pantoea sp. , Microbacterium sp. , Curtobacterium sp. , Agrobacterium sp. , Pseudomonas sp. , or Bacillus sp. Among them, the Pseudomonas sp. was the

Effects of different chemotypes and seasonal dynamic variation on the species diversity of endophytic fungal communities harbored in Atractylodes lancea.

The effects of the addition of Ceratobasidum stevensii B6 and its growth on the soil microflora at a continuously cropped watermelon (Citrullus lanatus) site in China. Acta Ecologica Sinica,2012,32(4) :1185鄄1192.

Beauveria bassiana is a well鄄known entomopathogenic fungi with worldwide distribution that can be used as a microbial pesticide. To date, studies of B. bassiana have focused on its insecticidal mechanism, symbiosis with plants and antagonism of plant pathogens; however, few studies of its influence on the microecological phyllosphere and at physiological level of the plant have been conducted. To investigate the ecological security of B. bassiana in the paddy phyllosphere, we evaluated the effects of different concentrations of B. bassiana spore suspensions and a chemical pesticide ( acephate) on paddy phyllosphere microbial flora and protective enzyme activities by a potted trial. B. bassiana were then induced to express the egfp gene ( green fluorescent protein gene) through transformation with a vector, after which real鄄 time polymerase chain reaction ( PCR ) was used to quantify the organisms and measure their dynamics in a paddy http: / / www.ecologica.cn phyllosphere. We also evaluated the effects of different concentrations of B. bassiana suspensions on the phyllosphere microflora using DGGE. To accomplish this, B. bassiana specific DNA primers were designed based on the green fluorescent protein sequence marked B. bassiana. Amplification of B. bassiana DNA using the eGPF鄄F1 / eGFP鄄R1 primers yielded a single 289bp鄄long product with a detection limit of 10fg / 滋L of B. bassiana genomic DNA. The pot experiments, which were conducted in the botanical garden of Nanjing Normal University, consisted of the following seven treatments: sterile water applied as a control ( CK) , inoculation with the larva of Chilo suppressalis ( A) , application of the B. bassiana spore suspension at 7.5伊10 4 spores / ml ( B) , 7.5伊 10 5 spores / ml ( C) , 7.5伊 10 6 spores / ml ( D) , or 7.5 伊 10 7 spores / ml ( E) , application of acephate emulsifiable concentrates ( F) . Each treatment group was covered with gauze (3 m 伊 0.7 m 伊 1.5 m) after treatment. Fluorescence quantitative PCR analysis of the rice phyllosphere DNA revealed that B. bassiana was maintained for at least 30 days on the leaves of paddy plants after application, but when a higher initial dosage of the B.bassiana was applied, the B. bassiana population on the phyllosphere decreased more rapidly. When compared with chemical pesticide, B. bassiana did not significantly affect the antioxidant activity of three enzymes in paddy leaves.Specifically, the enzymatic activities of SOD and POD in B. bassiana treatment groups were 20. 38 and 8. 65% higher, respectively, than those in the CK group on the tenth day, while the activity of CAT was 33.67% higher than that of the CK group on day 30. However, the enzymatic activity of CAT in the F group was 42.71% lower than that of the CK group on day 10. DGGE cluster analysis showed that B. bassiana did not significantly influence the bacterial or fungal community structures on the paddy phyllosphere, and the microbial community structure similarity, Shannon index and band number in the B. bassiana treated group were higher than ...

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