OC-Hemocatch', an immunochromatographic test for the detection of fecal occult blood was evaluated for the forensic identiˆcation of human blood.`OC-Hemocatch' showed positive results for human blood to a dilution of 1:500,000 and provides the strongest detection line for a dilution of 1:20,000. On the other hand, the human blood diluted to 1:100 was negative for`OC-Hemocatch' because of the high dose hook eŠect. While heating at over 150°C, long term exposing to sunlight, washing and bleaching of bloodstains prevented the detecting of human hemoglobin using`OC-Hemocatch', contamination of blood with various body ‰uids did not aŠect it. Furthermore,`OC-Hemocatch' detected human hemoglobin from old bloodstains stored for 15 years at room temperature when 5 ammonia was used for extraction. These results demonstrate that`OC-Hemocatch' can be eŠectively applied to forensic identiˆcation of human blood.

We had already reported a speciˆcity of the anti-A monoclonal antibodies (mAbs) that were produced by immunization of mice with salivary mucin obtained from blood group A secretors and non-secretors. In this report, an anti-A mAb K7405 reacting to the saliva from secretor, and a K7516 anti-A mAb reacting to the saliva from both secretor and non-secretor were applied to forensic investigation. In the forensic blood typing of saliva, blood group A secretors and blood group A nonsecretors can be clearly discriminated by the combined application of K7405 and K7516 for an absorption test, enzyme-linked immunosorbent assay and mixed cell agglutination reaction. However, the two mAbs could not completely discriminate between secretors and non-secretors from seminal samples.