Stable carbon isotope composition (啄 13 C value) was one of the effective methods for measuring the plant water use efficiency (WUE) ,and was already applied in many plants. At present, the study on estimation of the WUE of Cassia obtusifolia L.. by 啄 13 C was a few. In order to prove the reliability of 啄 13 C on measuring WUE for Cassia obtusifolia L., 啄 13 C、 stable carbon isotope discrimination (驻 13 C) 、WUE and the relationship among them are measured in different organs (root, steam, leaf) and different growth stages by pot experiment and 啄 13 C. The results showed that, the 啄 13 C value and 驻 13 C of roots were highest, the next were stems, leaves were minimum, there were significant level in the 啄 13 C value and 驻 13 C in roots in different growth stages, positive relationship were found between the 啄 13 C value and WUE in stems and leaves, negative relationship were found between the 驻 13 C and WUE in stems and leaves. It showed significant positive correlation level among WUE (Potted method) and WUE L 、WUE S 、WUE R (Carbon isotope method) , related coefficient were 0. 86、 0郾 82、0.80 respectively, those showed carbon isotope technology was reliable to determine the WUE of Cassia obtusifolia L..
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