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The objective of this study was to compare two extraction methods for determination of vitamin K1 (phylloquinone) in vegetables. In addition, analytical method validation parameters such as accuracy, precision, limit of detection (LOD), limit of quantification (LOQ), and linearity were calculated to ensure the method's validity. Vitamin K1 was quantified by reversed-phase HPLC using post-column derivatization and fluorescence detection (Exλ=243 nm, Emλ=430 nm). Higher analytical values were observed using solvent extraction compared to those from the enzyme extraction method. The results from the method validation showed high linearity in the calibration curve with a coefficient of correlation (R 2 ) of 0.9994. The LOD and LOQ were 0.1335 and 0.2784 ng/injection volume (50 μL), respectively. The inter-day precision and inter-day precision were 2.0% and 2.1%, respectively. Overall recovery was close to 100% (n=5). The phylloquinone contents ranged from 9.42 to 1,212.57 μg/100 g. Our study provides reliable data on the phylloquinone contents in commonly consumed vegetables in Korea.