The nitrogen source-requirement and preference of ectomycorrhizal fungi (42 strains belong to 13 genera) collected from Korean forests were investigated by incubating mycelial cells in four nitrogen source test media for 56 days. The nitrogen requirement was relatively high in five strains (Heimioporus japonicas (KFRI 1224), Suillus luteus (KFRI 1232), Tylopilus castaneiceps (KFRI 1383), Suillus granulates (KFRI 1997), Rhizopogon sp. (KFRI 1434)) in comparison with other strains. Forty-five percent of ectomycorrhizal strains including Boletus griseus (KFRI 1362) preferred the ammonium-form of inorganic nitrogen source for mycelial growth, whereas thirty-six percent of tested strains including Suillus grevillei (KFRI 1125) preferred the nitrateform as an inorganic nitrogen source.
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