서 론Metagenomics를 이용한 미생물상 분석법은, 다양한 토양 미생물의 대량 분석을 가능하게 했을 뿐 아니라. 배양이 불 가능한 미생물의 분포 조사에 이용되고 있다 (Amann et al., 1995). 현재 사용되고 있는 미생물 분류 및 동정 방법은 생 화학적 방법으로 미생물의 특성을 검정하거나 rRNA의 염 기서열을 분석하는 분자생물학적 방법으로 이루어지고 있 다 (Woese and Fox, 1977;Woese et al., 1990 ABSTRACT. Large patch, caused by Rhizoctonia solani AG2-2 IV, is a soil-born disease that is the most important of warm season turfgrass such as zoysia and Bermuda grass. This study was conducted to analysis of the soil microbial community structure on large patch. Center of the large patch (CLC), edge (CLE) and healthy (CLH) part of microbial communities were examined using metagenomics in Phylum level. Distribution trends of the rhizosphere microorganisms were similar to the order Proteobacteria, Acidobacteria, Chloroflexi, Firmicutes, Planctomycetes, Gemmatimonadetes, Nitrospira, Cyanobactria and Verrucomicrobia in soil collections. Contrastively Actinobacteria was more 56% abundant in healthy part soil (16%) than in the center (9.28%) or edge (10.84%) parts. Taxonomic distributions were compared among the CLC, CLE and CLH, total 6,948 OTUs were detected in the CLC, 6,505 OTUs for the CLE and 5,537 OTUs were detected in the CLE. Distributions of Actinobacteria OTUs were appeared 615 OTUs in the CLC, 709 OTUs in the CLE and 891 OTUs in the CLH. Among Actinobacteria, 382 OTUs were overlapped in the all soils. Not matched OTUs of CLH (286 OTUs) was detected 23 times higher than CLC (91 OTUs) and CLE (126 OTUs).

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Abstract. Stauntonia hexaphylla and Raphiolepis indica, cold-tolerant broadleaved evergreens ranging through the southern region of South Korea, were assessed on salt tolerance with NaCl treatment using visual damage, chlorophyll florescence image, and malondialdehyde (MDA) analysis. As NaCl concentrations increased, the soil pH decreased and EC increased, and the soil of S. hexaphylla was affected more strongly by the treatment than that of R. indica. In visual damage, S. hexaphylla withered above 200 mM NaCl at 20 days after the treatment. All individuals of R. indica survived during the experiment though the leaves of R. indica showed visual damages up to 400 mM NaCl. The color changes in chlorophyll fluorescence showed a strong correlation with the degree of visual damage. As NaCl increased, the red color of the leaves of S. hexaphylla was distinctly changed to blue and chlorophyll fluorescence decreased starting from the margin to the middle of a leaf. R. indica showed subtle color changes and remained in red color during the experiment. At five days after the NaCl treatment, the MDA of S. hexaphylla was above 4.56 nmol･g -1 when plants showed the highest visual damage and EC. The MDA of R. indica in all treatments showed below 1.5 nmol･g -1 except 400 mM NaCl treatment during the experiment.

잔디는 빈번한 깎기 조건에서 계속적인 생장이 이루어지 기 때문에 다른 작물에 비해 영양 요구도가 높으며, 이러 한 요구를 충족시키는 화학비료 중심의 시비관리 결과 칼 슘과 마그네슘 같은 생장필수양분의 결핍으로 토양의 영 양불균형을 초래될 수 있다 (Ham et al., 1996;Hwang and Choi, 1999 (Bae et al., 2013c;Kim et al., 2003;Joo and Lee, 2011).규소는 식물의 필수원소는 아니지만 화본과 작물에 있어 그 효과가 입증되어 왔으며, 표피세포와 목질부 세포벽에 규소가 축적되어 세포벽의 견고성을 높이고 (Epstein, 1994;Mengel and Krikby, 1987), 잎의 크기와 두께의 생장을 촉 ABSTRACT. Silicate fertilizers known to be effective in improving the growth and density of zoysiagrass. Most silicate fertilizers being used in Korea are slag-originated silicate fertilizer, but some water soluble silicate fertilizers are commercially available recently. This study was conducted to know the effect of water soluble silicate fertilizer, on the growth of zoysiagrass and the change of soil chemical properties in Wagner pot and field experiment. Root length, fresh and dry weight of shoots and stolons, the number of shoots and stolons, total of stolons length and the SiO 2 content of internal plant were significantly increased by the SiO 2 content but chemical properties of the soil were not significantly changed by the SiO 2 content. The SiO 2 contents of 18 and 36 µl ml -1 did not show significance difference, and therefore a reasonable application the content of SiO 2 was thought to be 18 µl ml -1 . Foliar spray of water soluble silicate fertilizer is believed to enhance the growth and density of zoysiagrass than soil application.