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Nardese, Vanessa; Longhi, Renato; Polo, Simona; Sironi, Francesca; Arcelloni, Cinzia; Paroni, Rita; DeSantis, Claudio; Sarmientos, Paolo; Rizzi, Menico; Bolognesi, Martino; Pavone, Vincenzo; Lusso, Paolo

doi:10.1038/89653

Cited by 48 publications

(16 citation statements)

References 27 publications

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“…However, several small–medium size peptides and many large peptides and/or proteins are still unavailable by classical methods. One example is RANTES, a 68 amino acid chemokine (Figure 1) that has a high propensity to aggregate 2, 3…”

Section: Introductionmentioning

confidence: 99%

The synergy of ChemMatrix resin® and pseudoproline building blocks renders Rantes, a complex aggregated chemokine

García-Martín

White

Steinauer³

et al. 2006

Biopolymers

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Traditionally, solid-phase synthesis has relied on polystyrene-based resins for the synthesis of all kinds of peptides. However, due to their high hydrophobicity, these resins have certain limitations, particularly in the synthesis of complex peptides, and in such cases, poly(ethylene glycol) (PEG)-based resins are often found to give superior results. Another powerful strategy for expediting the assembly of complex peptides is to employ pseudoproline dipeptides. These derivatives disrupt the interactions among chains that are usually the cause of poor coupling yields in aggregated sequences. Here we report on an efficient stepwise solid-phase synthesis of RANTES (1-68) by combining the advantages of the totally PEG-based ChemMatrix resin and pseudoproline dipeptides.

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Section: Introductionmentioning

confidence: 99%

The synergy of ChemMatrix resin® and pseudoproline building blocks renders Rantes, a complex aggregated chemokine

García-Martín

White

Steinauer³

et al. 2006

Biopolymers

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“…Similarly, several small CCR5 antagonists have been reported to block HIV-1 infection 100–102 (Table 2). TAK-779, a non-peptide compound, binds to CCR5 primarily via polar interactions with Glu 283 and Tyr 37 , hydrophobic interactions with Phe 10 , Phe 112 , Phe 113 , Ile 198 and Trp 248 deeply buried in the TM regions, and a face-to-face aromatic stacking contact with Tyr 108 103,104 .…”

Section: Chemokine Receptor Inhibitorsmentioning

confidence: 99%

Biology and clinical relevance of chemokines and chemokine receptors CXCR4 and CCR5 in human diseases

Choi

2011

Exp Biol Med (Maywood)

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Chemokines and their receptors are implicated in a wide range of human diseases, including acquired immune deficiency syndrome (AIDS). The entry of human immunodeficiency virus type 1 (HIV-1) into a cell is initiated by the interaction of the virus’s surface envelope proteins with two cell surface components of the target cell, namely CD4 and a chemokine co-receptor, usually CXCR4 or CCR5. Typical anti-HIV-1 agents include protease and reverse transcriptase inhibitors, but the targets of these agents tend to show rapid mutation rates. As such, strategies based on HIV-1 co-receptors have appeal because they target invariant host determinants. Chemokines and their receptors are also of general interest since they play important roles in numerous physiological and pathological processes in addition to AIDS. Therefore, intensive basic and translational research is ongoing for the dissection of their structure – function relationships in an effort to understand the molecular mechanism of chemokine – receptor interactions and signal transductions across cellular membranes. This paper reviews and discusses recent advances and the translation of new knowledge and discoveries into novel interventional strategies for clinical application.

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“…RANTES has also been shown to possess antiviral properties. Treatment with recombinant CCL5 or with synthetic peptides representing the N-terminal loop of CCL5 inhibited infection of human monocyte cultures by a CCR5-dependent human immunodeficiency virus strain [12]. Pretreatment with biologically inactive met-CCL5 also significantly inhibited infection of human epithelial cells with respiratory syncytial virus [13].…”

Section: Introductionmentioning

confidence: 94%

Foot-and-mouth disease virus leader proteinase inhibits dsRNA-induced RANTES transcription in PK-15 cells

Wang

Fang

et al. 2011

Virus Genes

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The chemokine RANTES (regulated upon activation, normal T-cells expressed and secreted) plays an essential role in inflammation and immune response. Infection with wild-type foot-and-mouth disease virus (FMDV) in PK-15 cells strongly inhibits the expression of RANTES compared to infection with a genetically engineered mutant lacking the leader protein (L(pro)) coding region. This suggests that L(pro) is involved in RANTES regulation. However, the underlying molecular mechanism remains unclear. In this study, we show that transfection of PK-15 cells with a plasmid expressing the L(pro) of FMDV, in the absence of other FMDV proteins, inhibited dsRNA-induced RANTES transcription and promoter activity. Promoter mutagenesis experiments revealed that the interferon-stimulated response element (ISRE) was important for the ability of L(pro) to inhibit dsRNA-induced RANTES promoter activity. Furthermore, over-expression of L(pro) also inhibited IRF-3/7-mediated RANTES activation. Screening L(pro) mutants indicated that catalytic activity and a SAP (for SAF-A/B, Acinus, and PIAS) domain of L(pro) were required to suppress dsRNA-induced RANTES transcription.

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Untitled

Cited by 48 publications

References 27 publications

The synergy of ChemMatrix resin® and pseudoproline building blocks renders Rantes, a complex aggregated chemokine

The synergy of ChemMatrix resin® and pseudoproline building blocks renders Rantes, a complex aggregated chemokine

Biology and clinical relevance of chemokines and chemokine receptors CXCR4 and CCR5 in human diseases

Foot-and-mouth disease virus leader proteinase inhibits dsRNA-induced RANTES transcription in PK-15 cells

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