03-P087 SHH patterning effects on chicken pretectal region between stages HH13 and HH24

Abstract

the Fgf8b mouse isoform. The cell line HEK-293 was transfected with this construct to obtain a permanent clone cell line in which the Fgf8b-EGFP could be secreted on the extracellular matrix. The inductive activity of Fgf8b-EGFP protein was proved either in vitro (cell cultures) as in ex-vivo (organotypic explant cultures of E9.5