1,25(OH)2D3 and VDR Signaling Pathways Regulate the Inhibition of Dectin-1 Caused by Cyclosporine A in Response to Aspergillus Fumigatus in Human Corneal Epithelial Cells

Xia, Yiping; Zhao, Guiqiu; Lin, Jing; Cui, Li; Lin, Cong; Jiang, Nan; Xu, Qiang; Wang, Qian

doi:10.1371/journal.pone.0164717

Cited by 6 publications

(3 citation statements)

References 39 publications

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“…The inactive A. fumigatus hyphae (to a final concentration of 5×10 6 CFU/mL) were prepared as per Xia et al and added to the cell culture for stimulation of dectin 1. 23…”

Section: Cell Culture and A Fumigatus Stimulationmentioning

confidence: 99%

Preparation of a Nanobody Specific to Dectin 1 and Its Anti-inflammatory Effects on Fungal Keratitis

Liu¹,

Sui²,

Li³

et al. 2022

IJN

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To prepare a nanobody specific to dectin 1 and verify its specificity and anti-inflammatory effects on Aspergillus fumigatus keratitis. Methods: The nanobody was selected from a high-quality shark-antibody library constructed with phage-display technology. The nanobody was developed in the expression systems of Escherichia coli. Indirect ELISA was used to determine the specificity of the nanobody to recombinant dectin 1 protein. The potential of the nanobody to be recognized and expressed on the surfaces of cells and corneas was detected by immunofluorescence, and its anti-inflammatory effect on A. fumigatus keratitis was further verified. After infection with A. fumigatus, eyes of C57B L/6 mice were treated with nanobodies. Human corneal epithelial cells (HCECs) were pretreated with nanobodies and then incubated with A. fumigatus. Clinical scores and slit-lamp photography were used to assess disease response in mouse corneas. RT-PCR and ELISA were used to evaluate mRNA and protein expression of IL1β and IL6 in both mouse corneas and HCECs. Results: The nanobody was successfully expressed through microbial system and showed specific high-affinity binding to recombinant dectin 1. Furthermore, it exhibited specific binding to dectin 1 expressed on the surfaces of cells and recognized dectin 1 in mouse corneas. Importantly, it reduced clinical scores of A. fumigatus keratitis in mice compared with a PBS-treatment group. In addition, it decreased mRNA and protein expression of IL1β and IL6 in infected corneas and HCECs stimulated with A. fumigatus. Conclusion: These results suggest that this nanobody can bring about anti-inflammatory effects. This highlights the potential of these nanobodies as innovative therapeutic agents in A. fumigatus.

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“…The inactive A. fumigatus hyphae (to a final concentration of 5×10 6 CFU/mL) were prepared as per Xia et al and added to the cell culture for stimulation of dectin 1. 23…”

Section: Cell Culture and A Fumigatus Stimulationmentioning

confidence: 99%

Preparation of a Nanobody Specific to Dectin 1 and Its Anti-inflammatory Effects on Fungal Keratitis

Liu¹,

Sui²,

Li³

et al. 2022

IJN

Self Cite

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“…128 The relationship of TLR4 with DR-driven repair mechanisms has been investigated by Vespasiani-Gentilucci et al, 129 showing a close correlation between TLR4 expression on HPC and DR cells and the inflammatory cell infiltrate, and deposition of fibrosis in liver biopsies of 74 patients with NAFLD. It is particularly noteworthy that TLR4 signaling is modulated by the vitamin D/Vitamin D receptor (VDR) pathway, acting as a fundamental protective mechanism not only in the liver, but even in the oral epithelium, 130 cornea, 131 and pancreas. 132 Similarly, reduced serological levels of 25-hydroxy-vitamin D3 and reduced tissue expression of VDR in both hepatocytes and cholangiocytes have been reported in both NAFLD 133 or NASH.…”

Section: Tlrsmentioning

confidence: 99%

The Neglected Role of Bile Duct Epithelial Cells in NASH

et al. 2021

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Nonalcoholic fatty liver disease (NAFLD) is the most prevalent liver disease worldwide, and affects 25% of the population in Western countries. NAFLD is the hepatic manifestation of the metabolic syndrome, linked to insulin resistance, which is the common pathogenetic mechanism. In approximately 40% of NAFLD patients, steatosis is associated with necro-inflammation and fibrosis, resulting in nonalcoholic steatohepatitis (NASH), a severe condition that may progress to cirrhosis and liver cancer. Although the hepatocyte represents the main target of the disease, involvement of the bile ducts occurs in a subset of patients with NASH, and is characterized by ductular reaction and activation of the progenitor cell compartment, which incites portal fibrosis and disease progression. We aim to dissect the multiple biological effects that adipokines and metabolic alterations exert on cholangiocytes to derive novel information on the mechanisms driven by insulin resistance, which promote fibro-inflammation and carcinogenesis in NASH.

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“…No entanto, diferenças entre gêneros, grupos étnicos e subtipos potencialmente diferentes dessa doença tornam difícil decifrar o papel exato da vitD na mesma. Além disso, embora a suplementação de vitD tenha sido bem-sucedida na melhora dos sintomas em alguns estudos, isso não foi demonstrado de forma consistente (XIA et al, 2016).…”

Section: Propriedades Imunomoduladoras Da Vitamina Dunclassified

Estudo comparativo do mecanismo de ação da Vitamina D na prevenção da artrite reumatóide e psoríase / Comparative study of the mechanism of action of Vitamin D in the prevention of rheumatoid arthritis and psoriasis

Batista¹,

Junior²,

Martim³

2021

Braz. J. Hea. Rev.

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A vitamina D (VitD) (colecalciferol) é uma molécula esteroide solúvel em gordura que é obtida tanto da ingestão alimentar quanto da síntese endógena, a deficiência de VitD está correlacionada com muitas condições clínicas e fisiopatológicas, bem como com o desenvolvimento e exacerbação de várias doenças autoimunes. Do mesmo modo, tem havido um interesse crescente em elucidar a relação entre VitD e a imunidade. Dessa forma, este estudo teve por objetivo realizar uma revisão da literatura a fim de descrever o mecanismo de ação da vitamina D na prevenção da artrite reumatoide e psoríase. O estudo foi realizado por meio dos bancos de dados: Google Acadêmico, Scielo -Scientific Electronic Library Online, Lilacs -Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde, Pubmed -U. S. National Library of Medicine executando a busca do material que foi pesquisado por meio dos descritores: Vitamina D, Artrite Reumatoide, Psoríase e Doenças autoimunes. Estes termos foram utilizados em português e inglês no período de 2010 a 2021. Dado o papel proposto para a vitD como um regulador imunológico, talvez não seja surpreendente que a deficiência de vitD tenha sido associada a doenças alérgicas e autoimunes. Esta revisão elucidou o papel da vitamina-D na prevenção de doenças autoimunes. Como observado, foi descrito o metabolismo de formação da vitD, assim como os mecanismos de atuação na artrite reumatoide, psoríase e doença de Crohn.

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1,25(OH)2D3 and VDR Signaling Pathways Regulate the Inhibition of Dectin-1 Caused by Cyclosporine A in Response to Aspergillus Fumigatus in Human Corneal Epithelial Cells

Cited by 6 publications

References 39 publications

Preparation of a Nanobody Specific to Dectin 1 and Its Anti-inflammatory Effects on Fungal Keratitis

Preparation of a Nanobody Specific to Dectin 1 and Its Anti-inflammatory Effects on Fungal Keratitis

The Neglected Role of Bile Duct Epithelial Cells in NASH

Estudo comparativo do mecanismo de ação da Vitamina D na prevenção da artrite reumatóide e psoríase / Comparative study of the mechanism of action of Vitamin D in the prevention of rheumatoid arthritis and psoriasis

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