1-Naphthol as a Sensitive Fluorescent Molecular Probe for Monitoring the Interaction of Submicellar Concentration of Bile Salt with a Bilayer Membrane of DPPC, a Lung Surfactant

Mohapatra, Manoranjan; Mishra, Ashok Kumar

doi:10.1021/jp103855q

Cited by 43 publications

(75 citation statements)

References 44 publications

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“…Such blue shifts are known to occur in the microenvironments of organized assemblies. 23 The concomitant enhancement of the NpOH* emission clearly indicates that the observed non-polarity is due to the non-availability of proton-receiving water in the microenvironment, which results in the retardation of the ESPT rate. The presence of an isoemissive region further confirms the existence of a two-state equilibrium ( Fig.…”

Section: Resultsmentioning

confidence: 97%

“…In water, the fluorescence lifetime of NpO À * at 460 nm is 8 ns and the fluorescence lifetime of NpOH* at 360 nm is 35 ps. 20,23 In PF127 solution, the NpO À * fluorescence decay has a biexponential fit until 20 1C; above 20 1C, it shows a triexponential fit with a rise time of 3-4 ns (Fig. 2(A), Table 1, ESI, † Fig.…”

Section: Fluorescence Lifetime Studiesmentioning

confidence: 99%

“…23 Thus, this component can be ascribed to the micellar core region in the PF127 sol state and the dry non-polar core of the gel state. The NpOH* emission lifetimes of o5 ns imply the availability of proton-receiving water around the molecule.…”

Section: Fluorescence Lifetime Studiesmentioning

confidence: 99%

“…These features have been well exploited in a recent study by Mohapatra et al which found that the incorporation of bile salt molecules in the bilayer membrane significantly hydrates the membrane, even extending to the core of the membrane. 23 Thus, monitoring the thermotropic structural changes of an aqueous PF127 system using NpOH as an ESPT fluorescent molecular probe is expected to provide a clearer understanding of the nature of the hydration in the microenvironments around the probe in different states of thermotropic aggregation. This is the objective of this work.…”

Section: Introductionmentioning

confidence: 99%

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1-Naphthol as an ESPT fluorescent molecular probe for sensing thermotropic microenvironmental changes of pluronic F127 in aqueous media

Swain

Mishra

2015

Phys. Chem. Chem. Phys.

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Thermotropic microenvironmental changes and the level of hydration in different microenvironments of pluronic F127 (PF127), (PEO106 PPO70 PEO106, average molar mass 13 000) in aqueous media have been studied using 1-naphthol, which is an ESPT fluorescent molecular probe. The appearance of 1-naphthol neutral form fluorescence in aqueous PF127 (10% w/v) solution indicates the ability of 1-naphthol to sense hydrophobic domains in micellar aggregations. There is a marked enhancement of the neutral form fluorescence at and above the gelation temperature (20 °C), which shows that the probe can accurately sense the sol-gel transition. In the temperature range of 10-40 °C, with increase in temperature there is a progressive enhancement of the neutral form fluorescence and the blue shift of the neutral and anionic form fluorescence; a decrease in the deprotonation rate constant (kpt) indicates that the water-polymer interfacial region is progressively dehydrated. Because kpt is related to the availability of proton-accepting water in the microenvironment of 1-naphthol, the reduction of kpt indicates progressive dehydration. The thermotropic response of the I1/I3 vibronic band ratio of pyrene-1-butyric acid fluorescence shows a progressive increase in the non-polarity of the interfacial domain with increasing temperature. The increase in non-polarity and the decrease of the hydration level are strongly correlated.

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Section: Resultsmentioning

confidence: 97%

Section: Fluorescence Lifetime Studiesmentioning

confidence: 99%

Section: Fluorescence Lifetime Studiesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

1-Naphthol as an ESPT fluorescent molecular probe for sensing thermotropic microenvironmental changes of pluronic F127 in aqueous media

Swain

Mishra

2015

Phys. Chem. Chem. Phys.

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“…29,30 The usefulness of 1-naphthol as a molecular probe is reported through changes in its fluorescence parameters like intensity and lifetime. 33 Fig . Depending on the medium, 1-naphthol emits from two states, neutral and anion.…”

Section: Dmpc Lipid Bilayer Membrane Studiesmentioning

confidence: 99%

Study of aqueous phase aggregation of FTY720 (fingolimod hydrochloride) and its effect on DMPC liposomes using fluorescent molecular probes

Swain

Mohapatra

Borkar

et al. 2013

Phys. Chem. Chem. Phys.

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This work focuses on the study of aqueous phase aggregation of the recently FDA approved oral drug molecule FTY720 (fingolimod hydrochloride) and its effect on dimyristoylphosphatidylcholine (DMPC) liposomes using different fluorescent molecular probes and fluorescence parameters. The variation of the steady state fluorescence intensity of 8-anilino-1-naphthalene sulfonic acid (ANS) with FTY720 in water shows an efficient micellar aggregation with the critical micellar concentration (CMC) at ~75 μM. The aggregation number calculation from steady state fluorescence quenching of pyrene shows the formation of small micellar aggregates in aqueous solution having an aggregation number of 42 ± 3 with the free energy of micellization ~-23 kJ mol(-1). Fluorescence intensity and lifetime decay analysis of the molecular probe 1-naphthol indicate that the interaction of FTY720 with the DMPC lipid bilayer membrane prevents partitioning of small molecules such as 1-naphthol to the membrane in both solid gel (SG) and liquid crystalline (LC) phases. Temperature dependent fluorescence intensity studies of 1-naphthol and fluorescence anisotropy measurements of 1,6-diphenyl-1,3,5-hexatriene (DPH) have shown that above the CMC of FTY720, the SG to LC main phase transition temperature (T(M)) of the lipid bilayer membrane decreases from 23 °C to 21 °C in the aqueous medium.

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Modulation of Excited‐State Proton‐Transfer Dynamics inside the Nanocavity of Microheterogeneous Systems: Microenvironment‐Sensitive Förster Energy Transfer to Riboflavin

et al. 2019

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The excited‐state proton‐transfer efficiency of a tetraarylpyrene derivative, 1,3,6,8‐tetrakis(4‐hydroxy‐2,6‐dimethylphenyl)pyrene (TDMPP), was investigated thoroughly in the presence of various surfactant assemblies, such as micelles and vesicles. The confined microheterogeneous environments can significantly retard the extent of the excited‐state proton‐transfer process, resulting in a distinguishable optical signal compared to that in the bulk medium. Physical characteristics of the surfactant assemblies, such as order, interfacial hydration, and surface charge, influence the proton transfer process and allow multiparametric sensing. A higher degree of interfacial hydration facilitates the proton‐transfer process, while the positively charged head groups of the surfactants specifically stabilize the anionic form of the probe (TDMPP−O*). Furthermore, Forster energy transfer from the probe to riboflavin was studied in a phospholipid membrane, wherein the relative ratio of the neutral versus anionic forms (TDMPP‐OH/TDMPP−O*) was found to influence the extent of energy transfer. Overall, we demonstrate how an ultrafast photophysical process, that is, the excited‐state proton transfer, can be influenced by the microenvironment.

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1-Naphthol as a Sensitive Fluorescent Molecular Probe for Monitoring the Interaction of Submicellar Concentration of Bile Salt with a Bilayer Membrane of DPPC, a Lung Surfactant

Cited by 43 publications

References 44 publications

1-Naphthol as an ESPT fluorescent molecular probe for sensing thermotropic microenvironmental changes of pluronic F127 in aqueous media

1-Naphthol as an ESPT fluorescent molecular probe for sensing thermotropic microenvironmental changes of pluronic F127 in aqueous media

Study of aqueous phase aggregation of FTY720 (fingolimod hydrochloride) and its effect on DMPC liposomes using fluorescent molecular probes

Modulation of Excited‐State Proton‐Transfer Dynamics inside the Nanocavity of Microheterogeneous Systems: Microenvironment‐Sensitive Förster Energy Transfer to Riboflavin

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